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Structure and Mechanism of the Lactose Permease of Escherichia coli
Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure atExpand
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Lessons from lactose permease.
  • L. Guan, H. Kaback
  • Chemistry, Medicine
  • Annual review of biophysics and biomolecular…
  • 11 May 2006
An X-ray structure of the lactose permease of Escherichia coli (LacY) in an inward-facing conformation has been solved. LacY contains N- and C-terminal domains, each with six transmembrane helices,Expand
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The kamikaze approach to membrane transport
Membrane transport proteins catalyse the movement of molecules into and out of cells and organelles, but their hydrophobic and metastable nature often makes them difficult to study by traditionalExpand
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Structure of sugar-bound LacY
Significance The lactose permease of Escherichia coli (LacY), a model for the major facilitator superfamily, catalyzes the symport of a galactopyranoside and an H+ across the membrane by a mechanismExpand
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Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and β-pancreatic islet cells
Abstract The well-characterized erythrocyte glucose transporter is also expressed in brain, adipocytes, kidney, muscle, and certain transformed cells, but not in liver, intestine, or the islets ofExpand
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Role of YidC in folding of polytopic membrane proteins
YidC of Echerichia coli, a member of the conserved Alb3/Oxa1/YidC family, is postulated to be important for biogenesis of membrane proteins. Here, we use as a model the lactose permease (LacY), aExpand
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Sugar binding induces an outward facing conformation of LacY
According to x-ray structure, the lactose permease (LacY) is a monomer organized into N- and C-terminal six-helix bundles that form a deep internal cavity open on the cytoplasmic side with a singleExpand
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[13] Bacterial Membranes
Publisher Summary This chapter discusses techniques used in the laboratory for both small- and large-scale preparation of bacterial membranes from various gram-positive and gram-negative organisms.Expand
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NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain.
Deamino-NADH/ubiquinone 1 oxidoreductase activity in membrane preparations from Escherichia coli GR19N is 20-50% of NADH/ubiquinone 1 oxidoreductase activity. In comparison, membranes from E. coliExpand
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Cys‐scanning mutagenesis: a novel approach to structure—function relationships in polytopic membrane proteins
The entire lactose permease of Escherichia coli, a polytopic membrane transport protein that catalyzes β‐galactoside/H+ symport, has been subjected to Cys‐scanning mutagenesis in order to determineExpand
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