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Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection…
Various 5'O-N-protected deoxynucleoside-3'-O-beta-cyanoethyl-N,N-dialkylamino-/N- morpholinophosphoramidites were prepared from beta-cyanoethyl monochlorophosphoramidites of N,N-dimethylamine,… Expand
Chip-based genotyping by mass spectrometry.
- K. Tang, D. Fu, D. Julien, A. Braun, C. Cantor, H. Köster
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 31 August 1999
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip… Expand
A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxyribonucleotide primers.
A new procedure for the production of a defined library of random mutants is described. Long spiked oligodeoxyribonucleotides (oligos), in which a predetermined level of the three 'wrong'… Expand
The use of MassARRAY technology for high throughput genotyping.
- C. Jurinke, D. van den Boom, C. Cantor, H. Köster
- Biology, Medicine
- Advances in biochemical engineering/biotechnology
This chapter will explore the role of mass spectrometry (MS) as a detection method for genotyping applications and will illustrate how MS evolved from an expert-user-technology to a routine… Expand
A comparison of acid labile linkage agents for the synthesis of peptide C-terminal amides
Abstract Substituted benzhydrylamine and benzylamine linkage agents useful for the solid phase peptide synthesis of C-terminal amides were evaluated for their relative lability toward trifluoroacetic… Expand
MALDI on a Chip: Analysis of Arrays of Low-Femtomole to Subfemtomole Quantities of Synthetic Oligonucleotides and DNA Diagnostic Products Dispensed by a Piezoelectric Pipet
A piezoelectric pipet is used to dispense arrays of low-nanoliter aliquots of matrix and DNA into individual etched wells on <1 in.2 silicon chips prior to their semiautomated analysis by… Expand
Applications of dioxetane chemiluminescent probes to molecular biology.
DNA probes and synthetic oligonucleotides in general present one of the key tools in modern molecular biology research and increasingly also in commercial applications. Along with the many… Expand
β-Cyanoethyl N,N-dialkylamino/N-morpholinomonochloro phosphoamidites, new phosphitylating agents facilitating ease of deprotection and work-up of synthesized oligonucleotides
Abstract β-Cyanoethyl monochlorophosphoamidites of the secondary amines N,N-dmethylamine, N,N-diisopropylamine and morpholine have been prepared which showed quantitative phosphitylation of the… Expand
Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry.
A new method for the reliable identification of localized variations in DNA by detection of associated diagnostic products with matrix-assisted laser desorption ionization time-of-flight mass… Expand
Automated genotyping using the DNA MassArray technology.