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Adaptation of Pseudomonas aeruginosa to the cystic fibrosis airway: an evolutionary perspective
This work discusses how P. aeruginosa evolves from a state of early, recurrent intermittent colonization of the airways of patients with CF to a chronic infection state, and how this process offers opportunities to study bacterial evolution in natural environments. Expand
Convergent evolution and adaptation of Pseudomonas aeruginosa within patients with cystic fibrosis
The analysis of 36 P. aeruginosa lineages identified convergent molecular evolution in 52 genes that suggest a role in host adaptation for remodeling of regulatory networks and central metabolism, acquisition of antibiotic resistance and loss of extracellular virulence factors. Expand
Evolutionary dynamics of bacteria in a human host environment
The evolutionary dynamics of a lineage of a clinically important opportunistic bacterial pathogen, Pseudomonas aeruginosa, as it adapts to the airways of several individual cystic fibrosis patients is characterized, and estimates of mutation rates of bacteria in a natural environment are provided. Expand
Tolerance to the antimicrobial peptide colistin in Pseudomonas aeruginosa biofilms is linked to metabolically active cells, and depends on the pmr and mexAB‐oprM genes
It is demonstrated that the subpopulation of metabolically active cells in Pseudomonas aeruginosa biofilms is able to adapt to colistin by inducing a specific adaptation mechanism mediated by the pmr operon, as well as an unspecific adaptation mechanismmediated by the mexAB‐oprM genes. Expand
The clinical impact of bacterial biofilms
Bacterial biofilms are resistant to antibiotics, disinfectant chemicals and to phagocytosis and other components of the innate and adaptive inflammatory defense system of the body and they can be treated by chronic suppressive antibiotic therapy. Expand
Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Evaluated spa typing of methicillin-resistant Staphylococcus aureus isolates from new MRSA patients in Denmark found a 97% agreement between spa types obtained by the two methods, showing that WGS is a reliable method to determine the spa type of MRSA. Expand
Environmental Study of Azole-Resistant Aspergillus fumigatus and Other Aspergilli in Austria, Denmark, and Spain
ABSTRACT A single mechanism of azole resistance was shown to predominate in clinical and environmental Aspergillus fumigatus isolates from the Netherlands, and a link to the use of azoles in theExpand
Biofilms in chronic infections - a matter of opportunity - monospecies biofilms in multispecies infections.
The observations of the chronic biofilm infections point toward a trend of low bacterial diversity and sovereign monospecies biofilm aggregates even though the infection in which they reside are multispecies, while commensal and natural biofilm aggregation contain multiple species that are believed to coexist, interact and form biofilms with high bacterial and niche diversity. Expand
Novel Mouse Model of Chronic Pseudomonas aeruginosa Lung Infection Mimicking Cystic Fibrosis
The mouse model provides an improved method for evaluating the interaction between mucoid P. aeruginosa, the host, and antibacterial therapy and is similar to the clinical situation. Expand
Epidemic spread of Pandoraea apista, a new pathogen causing severe lung disease in cystic fibrosis patients
It is shown that Pandoraea apista must be added to the increasing list of pathogens capable of causing chronic lung infection in cystic fibrosis patients, and was transmissible among patients with CF, leading to spread of infection from the index patient to 5 patients exposed during participation in winter camps and/or hospitalization. Expand