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Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue Nanostructure
TLDR
It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits. Expand
The exocytotic event in chromaffin cells revealed by patch amperometry
TLDR
Exocytosis of individual chromaffin granules is investigated by using cell-attached capacitance measurements, combined with electrochemical detection of catecholamines, achieved by inserting a carbon-fibre electrode into the patch pipette and finding that the fusion-pore diameter stays at <3 nm for a variable period, which can last for several seconds, before it expands. Expand
Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells
TLDR
It is suggested that, temporally, the last ATP-dependent step in exocytosis is closely associated with docking and that docked granules reach fusion competence only after subsequent steps. Expand
Transport, docking and exocytosis of single secretory granules in live chromaffin cells
TLDR
It is concluded that a large pool of docked granules turns over slowly, that granules move actively to their docking sites, that docking is reversible, and that the ‘rapidly releasable pool’ measured electrophysiologically represents a small subset of docking granules. Expand
Regulation of Golgi structure and function by ARF-like protein 1 (Arl1).
TLDR
One of the mechanisms for the cell to regulate the structure and function of the Golgi apparatus is through the action of Arl1, which interacts with arfaptin-2/POR1 but not GGA1, both of which interact with GTP-restricted ARF1, suggesting that Arl 1 and ARf1 share some common effectors in regulating cellular events. Expand
Brain tumour cells interconnect to a functional and resistant network
TLDR
It is shown that many tumour cells in astrocytomas extend ultra-long membrane protrusions, and use these distinct tumour microtubes as routes for brain invasion, proliferation, and to interconnect over long distances, which develops functional multicellular network structures. Expand
Prenylation-dependent Association of Protein-tyrosine Phosphatases PRL-1, -2, and -3 with the Plasma Membrane and the Early Endosome*
TLDR
The results establish that the primary association of PRL-1, -2, and -3 with the membrane of the cell surface and the early endosome is dependent on their prenylation and that nuclear localization of these proteins may be triggered by a regulatory event that inhibits their preNylation. Expand
Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70.
TLDR
It is shown that a major 70 kd protein with an amino‐terminal hydrophobic membrane anchor and a hydrophilic 60 kd domain exposed to the cytosol accelerates the mitochondrial import of many (but not all) precursor proteins. Expand
β-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo
TLDR
In vivo evidence is provided that beta-COP has an important function in biosynthetic membrane traffic in mammalian cells by blocking transport of a temperature-sensitive vesicular stomatitis virus glycoprotein (ts-O45-G) to the cell surface. Expand
Immunocytochemical characterization of the endocytic and phagolysosomal compartments in peritoneal macrophages
TLDR
The data argue first that the PLC is equivalent to the "tubular lysosomes" commonly described in macrophages, and second that the meeting of the phagocytic and endocytic pathway occurs in this compartment. Expand
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