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High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation.
TLDR
It is reported that a complete deamination of cytosine to uracil can be achieved in shorter periods by using a highly concentrated bisulfite solution at an elevated temperature.
HAM1, the gene controlling 6‐N‐hydroxylaminopurine sensitivity and mutagenesis in the yeast Saccharomyces cerevisiae
TLDR
A clone from a centromere‐plasmid‐based genomic library complementing HAP sensitivity of the ham1 strain was isolated and the deduced amino acid sequence of HAM1 protein was not similar to any protein sequence of the SwissProt database.
Oxidative damage and induced mutations in m13mp2 phage DNA exposed to N-nitrosopyrrolidine with UVA radiation.
TLDR
The formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in a replicative form of M13mp2 DNA exposed to NPYR plus UVA may contribute to mutagenesis by NPYRplus UVA.
The cleavage of DNA by the oxygen-dependent reaction of bisulfite.
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