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Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery
TLDR
The different uptake pathways that are involved in nonviral gene delivery from a gene delivery point of view are reviewed and available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationsic polymer- DNA complexes (polyplexes) is summarized. Expand
The RNA sensor RIG-I dually functions as an innate sensor and direct antiviral factor for hepatitis B virus.
TLDR
Type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. Expand
Delivery of Macromolecules Using Arginine-Rich Cell-Penetrating Peptides: Ways to Overcome Endosomal Entrapment
TLDR
Different endosomal escape devices currently in application in combination with AR-CPPs are presented and their applications for the delivery of different macromolecules in vitro and, if applicable, in vivo are presented. Expand
Stearylated arginine-rich peptides: a new class of transfection systems.
TLDR
In the case of the Tat, FHV, and octaarginine peptides, N-terminal stearylation of the peptides increases the transfection efficiency by approximately 100 times to reach the same order of magnitude as that of LipofectAMINE, one of the most efficient commercially available transfections agents. Expand
Liposome Clearance
TLDR
The role of serum proteins, so-called opsonins, that enhance the clearance of liposome clearance, when bound to liposomes, is focused on. Expand
High Density of Octaarginine Stimulates Macropinocytosis Leading to Efficient Intracellular Trafficking for Gene Expression*
TLDR
The results show that the density of the R8 peptide on liposomes determines the uptake mechanism and that this is directly linked to intracellular trafficking, resulting in different levels of gene expression. Expand
A multifunctional envelope type nano device (MEND) for gene delivery to tumours based on the EPR effect: a strategy for overcoming the PEG dilemma.
TLDR
The development and applications of MEND are described, and strategies for overcoming the PEG dilemma are discussed, based on the manipulation of intracellular trafficking of cellular uptake and endosomal release using functional devices such as specific ligands, cleavable PEG systems andendosomal fusogenic/disruptic peptides. Expand
Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway. Expand
Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid
TLDR
In vivo studies revealed that the PPD was potent in stabilizing MEND in the systemic circulation and facilitating tumor accumulation, and MEND modified with PPD is a promising device, which has the potential to make in vivo cancer gene therapy achievable. Expand
Quantitative comparison of intracellular trafficking and nuclear transcription between adenoviral and lipoplex systems.
TLDR
A systematic and quantitative comparison of the cellular uptake and subsequent intracellular distribution of exogenous DNA transfected by viral and nonviral vectors in living cells is described, using a combination of TaqMan PCR and a recently developed confocal image-assisted three-dimensionally integrated quantification method. Expand
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