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Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1.
The new intracellular pH (pH(i)) dye carboxy-seminaphthorhodafluor (SNARF-1) was compared to the established dye 2,3-dicyanohydroquinone (DCH) using flow cytometry. Both dyes give high-resolution… Expand
Low pH suppresses synthesis of heat-shock proteins and thermotolerance.
Chinese hamster ovary (CHO) cells heated for 10 min at 45 degrees C became thermotolerant to a second heat exposure at either pH 7.3 or 6.6. However, low pH delayed the development of thermotolerance… Expand
Levels of 70-kDa heat shock protein through the cell cycle in several mammalian cell lines.
Seven different cell lines were analyzed by flow cytometry to evaluate the variation in heat shock protein hsp70 through the cell cycle. Inducible (hsp72) or both constitutive and inducible (hsp70)… Expand
Cell cycle variation of Hsp70 levels in HeLa cells at 37 degrees C and after a heat shock.
The expression of the 72 kD inducible heat shock protein (hsp72) has been reported to be cell cycle associated in unheated, synchronized HeLa cells. In this study, flow cytometry was used to… Expand
Differential contributions of NO3−/NH4+ to nitrogen use in response to a variable inorganic nitrogen supply in plantlets of two Brassicaceae species in vitro
BackgroundThe primary sources of nitrogen for plants have been suggested to be nitrate (NO3−) and ammonium (NH4+). However, when both nitrate and ammonium are simultaneously available to plants, it… Expand
Quantification of photosynthetic inorganic carbon utilisation via a bidirectional stable carbon isotope tracer
The amount of bicarbonate utilised by plants is usually ignored because of limited measurement methods. Accordingly, this study quantified the photosynthetic assimilation of inorganic carbon (CO2 and… Expand
Effect of Bicarbonate Stress on CarbonicAnhydrase Gene Expressions fromOrychophragmus violaceusand Brassica juncea seedlings
Three β-type genes coding for carbonic anhydrase and CA activities from Orychophragmus violaceus L. and Brassica juncea L. leaves in response to NaHCO3-induced bicarbonate stress were examined. Three… Expand
Biomass Production of Three Biofuel Energy Plants’ Use of a New Carbon Resource by Carbonic Anhydrase in Simulated Karst Soils: Mechanism and Capacity
To determine whether the bicarbonate in karst limestone soil could be used as a new carbon resource for biomass production by the catalysis of carbonic anhydrase (CA), a simulative karst drought… Expand
Expression of hsp70 induced in CHO cells by 45.0 degrees C hyperthermia is cell cycle associated and DNA synthesis dependent.
In response to a heat shock, the synthesis of heat shock proteins with a molecular weight of 72 kD (hsp72) is induced. To determine whether hsp72 expression in Chinese hamster ovary (CHO) cells is… Expand
The Adsorption-Flocculation Method Using Bentonite for Treatment of Heavy Metal-Containing Wastewater
The adsorption of Pb￣(2+), Cd￣(2+)and Cr￣(3+)ions respectively on the raw be ntonite and theconditions of flocculation of bentonite are studied in this paper.Combining the adsorption… Expand