Hydroxynitrile lyases : Functions and properties
- A. Hickel, M. Hasslacher, H. Griengl
- 1 December 1996
A general survey of the natural function and localization of this class of enzymes and a comprehensive summary of the biochemical and genetic data of the isolated proteins are presented.
High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.
- M. Hasslacher, M. Schall, H. Schwab
- BiologyProtein Expression and Purification
- 1 October 1997
Limitations in enzyme supply from natural resources were overcome by production of the enzyme in the microbial host systems Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris.
Threonine aldolases—an emerging tool for organic synthesis
- Johannes Steinreiber, Kateryna Fesko, H. Griengl
- Chemistry, Biology
- 22 January 2007
Oxidative biotransformations using oxygenases.
- Zhi Li, J. B. van Beilen, B. Witholt
- Biology, EngineeringCurrent Opinion in Chemical Biology
- 1 April 2002
Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis.
- U. Wagner, M. Hasslacher, H. Griengl, H. Schwab, C. Kratky
- 1 July 1996
Parameters influencing stability and activity of a S-hydroxynitrile lyase from Hevea brasiliensis in two-phase systems
- M. Bauer, H. Griengl, W. Steiner
- 1 June 1999
A biocatalytic Henry reaction--the hydroxynitrile lyase from Hevea brasiliensis also catalyzes nitroaldol reactions.
- Thomas Purkarthofer, K. Gruber, H. Griengl
- Chemistry, BiologyAngewandte Chemie
- 19 May 2006
This work examined the addition of nitromethane to aldehydes in the presence of the hydroxynitrile lyase from Hevea brasiliensis and considered replacing HCN by other nucleophiles to be added to carbonyl compounds catalyzed by these enzymes.
Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry
- Thomas Purkarthofer, W. Skranc, C. Schuster, H. Griengl
- Biology, ChemistryApplied Microbiology and Biotechnology
- 3 July 2007
Recently, protein engineering in combination with in silico methods gave rise to the development of a tailor-made HNL for large-scale manufacturing of a specific target cyanohydrin.
Four types of threonine aldolases: Similarities and differences in kinetics/thermodynamics
- Kateryna Fesko, C. Reisinger, Johannes Steinreiber, H. Weber, Martin Schürmann, H. Griengl
- Chemistry, Biology
- 1 June 2008