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Hydroxynitrile lyases : Functions and properties
A general survey of the natural function and localization of this class of enzymes and a comprehensive summary of the biochemical and genetic data of the isolated proteins are presented. Expand
Parameters influencing stability and activity of a S-hydroxynitrile lyase from Hevea brasiliensis in two-phase systems
Abstract ( S ) - hydroxynitrile lyase from Hevea brasiliensis (EC catalyzes the reversible formation of cyanohydrins from aldehydes of ketones and HCN. Stability and activity ofExpand
High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.
Limitations in enzyme supply from natural resources were overcome by production of the enzyme in the microbial host systems Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris. Expand
Oxidative biotransformations using oxygenases.
Preparative biotransformations have been carried out to synthesize useful pharmaceutical intermediates or chiral synthons on the gram to several-hundred-gram scale, by use of whole cells of wild type or recombinant strains. Expand
Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis.
The three-dimensional structure of hydroxynitrile lyase from the rubber tree, Hevea brasiliensis, is determined, revealing a tetrahedral hemiketal or hemiacetal intermediate formed by nucleophilic attack of Ser80 on the substrate, stabilized by the oxyanion hole. Expand
Threonine aldolases—an emerging tool for organic synthesis
In a systematic study, 21 ring-substituted benzaldehydes were reacted with glycine under catalysis with a l-threonine aldolase (lTA) from Pseudomonas putida and a d-threonine aldolase (dTA) fromExpand
A biocatalytic Henry reaction--the hydroxynitrile lyase from Hevea brasiliensis also catalyzes nitroaldol reactions.
This work examined the addition of nitromethane to aldehydes in the presence of the hydroxynitrile lyase from Hevea brasiliensis and considered replacing HCN by other nucleophiles to be added to carbonyl compounds catalyzed by these enzymes. Expand
Molecular Cloning of the Full-length cDNA of (S)-Hydroxynitrile Lyase from Hevea brasiliensis
Replacement of cysteine 81 by serine drastically reduced activity of the heterologous enzyme, suggesting a role for this amino acid residue in the catalytic action of Hnl. Expand
Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry
Recently, protein engineering in combination with in silico methods gave rise to the development of a tailor-made HNL for large-scale manufacturing of a specific target cyanohydrin. Expand