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Isolation of the Arabidopsis ABI3 gene by positional cloning.
The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone, and the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon.
High resolution two-dimensional electrophoresis of basic as well as acidic proteins
The broad host range pathogen Pseudomonas aeruginosa strain PA14 carries two pathogenicity islands harboring plant and animal virulence genes.
- Jianxin He, R. Baldini, L. Rahme
- BiologyProceedings of the National Academy of Sciences…
- 24 February 2004
Two P. aeruginosa pathogenicity islands (PAPI-1 and PAPI-2) are identified and characterized in the genome of PA14, a highly virulent clinical isolate, and a large set of "extra" virulence functions encoded by both PAPIs may contribute to the increased promiscuity of highly virulence P.aerug inosa strains, by directing additional pathogenic functions.
The Arabidopsis Abscisic Acid Response Locus ABI4 Encodes an APETALA2 Domain Protein
Arabidopsis abscisic acid (ABA)–insensitive abi4 mutants have pleiotropic defects in seed development, including decreased sensitivity to ABA inhibition of germination and altered seed-specific gene…
Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana
Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases, and 54% of the predicted genes had significant similarity to known genes, and other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, and the frequent occurrence of clustered gene families.
Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis.
- B. W. Shirley, W. Kubasek, H. Goodman
- Environmental ScienceThe Plant journal : for cell and molecular…
- 1 November 1995
Tissue- and locus-specific differences in the flavonols and anthocyanidins synthesized by mutant and wild-type plants are identified and evidence was uncovered that tt8 and ttg specifically affect dihydroflavonol reductase gene expression.
Isolation of the Arabidopsis GA4 locus.
Progeny from a transgenic Arabidopsis plant generated by the Agrobacterium root transformation procedure were found to segregate for a gibberellin (GA)-responsive semidwarf phenotype, and a higher level of transcription was detected in the ethyl methanesulfonate-induced ga4 mutant, and this overexpression was repressed by treatment with exogenous GA.
The Arabidopsis Photomorphogenic Mutant hy1 Is Deficient in Phytochrome Chromophore Biosynthesis as a Result of a Mutation in a Plastid Heme Oxygenase
- T. Muramoto, T. Kohchi, A. Yokota, I. Hwang, H. Goodman
- Biology, Environmental SciencePlant Cell
- 1 March 1999
Results indicate that the Arabidopsis HY1 gene encodes a plastid heme oxygenase necessary for phytochrome chromophore biosynthesis.
Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana
The sequence of chromosome 2 from the Columbia ecotype is reported in two gap-free assemblies (contigs) of 3.6 and 16 megabases, which represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date.
Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations.
Sequence analysis of the break points in both mutants indicate that repair of radiation-induced damage involves mechanisms similar or identical to those that mediate the integration of foreign sequences into the genome.