Apoprotein B100, an Inefficiently Translocated Secretory Protein, Is Bound to the Cytosolic Chaperone, Heat Shock Protein 70 (*)
- Mingyue Zhou, Xujun Wu, Li-Shin Huang, H. Ginsberg
- BiologyJournal of Biological Chemistry
- 20 October 1995
The results suggest that domains of nascent apoB localized on the C-terminal regions of the molecule are transiently exposed to the cytosol during translation and/or translocation, and that Hsp70 functions as a molecular chaperone to maintain apiB in a translocational competent conformation until translocation is completed.
Translocation Efficiency, Susceptibility to Proteasomal Degradation, and Lipid Responsiveness of Apolipoprotein B Are Determined by the Presence of β Sheet Domains*
- Jun-shan Liang, Xujun Wu, H. Ginsberg
- BiologyJournal of Biological Chemistry
- 25 December 1998
The results clearly demonstrate that the translocation efficiency, susceptibility to proteasomal degradation, and lipid responsiveness of apoB were determined by the presence of a lipid binding β sheet domain.
Demonstration of a Physical Interaction between Microsomal Triglyceride Transfer Protein and Apolipoprotein B during the Assembly of ApoB-containing Lipoproteins (*)
- Xujun Wu, Mingyue Zhou, Li-Shin Huang, J. Wetterau, H. Ginsberg
- Biology, ChemistryJournal of Biological Chemistry
- 26 April 1996
The results suggest that direct interaction between MTP and apoB occurs during the assembly of apOB-containing lipoproteins in HepG2 cells.
Evidence That a Rapidly Turning Over Protein, Normally Degraded by Proteasomes, Regulates hsp72 Gene Transcription in HepG2 Cells*
- Mingyue Zhou, Xujun Wu, H. Ginsberg
- Biology, ChemistryJournal of Biological Chemistry
- 4 October 1996
The results suggest that a rapidly turning over protein that is normally degraded by proteasomes may be involved in the regulation of Hsp70 synthesis via effects on the hsp70 transcriptional factor, HSF1.
A Two-site Model for ApoB Degradation in HepG2 Cells*
- Xujun Wu, N. Sakata, K. Lele, Mingyue Zhou, Hongshi Jiang, H. Ginsberg
- BiologyJournal of Biological Chemistry
- 25 April 1997
The results support the hypothesis that degradation of partially translocated apoB generates a 70-kDa amino-terminal fragment that is mainly degraded in the ER lumen by a DTT-sensitive pathway, and suggest that at least two distinct steps may be involved in the posttranslational degradation of apiB.
Enthalpies of transfer of transition states in the Menshutkin reaction from a polar protic to a dipolar aprotic solvent
- P. Haberfield, A. Nudelman, A. Bloom, R. Romm, H. Ginsberg
- Chemistry
- 1 July 1971
Electromechanical suppression of a demand pacemaker associated with electrode perforation.
- M. Rubenfire, H. Timmis, P. Freed, J. Evangelista, H. Ginsberg, W. Wajszczuk
- MedicineJournal of Electrocardiology
- 1973