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Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi
- Rasmus J. N. Frandsen, J. Andersson, M. Kristensen, H. Giese
- BiologyBMC Molecular Biology
- 1 August 2008
The new vectors designed for USER Friendly cloning provided a fast reliable method to construct vectors for targeted gene manipulations in fungi.
Identification of a gene cluster responsible for the biosynthesis of aurofusarin in the Fusarium graminearum species complex.
Chromosome Complement of the Fungal Plant Pathogen Fusarium graminearum Based on Genetic and Physical Mapping and Cytological Observations
A genetic map of the filamentous fungus Fusarium graminearum was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1, finding variation in recombination rate in different genomic regions that often spanned several hundred kilobases.
Nonribosomal peptide synthetase (NPS) genes in Fusarium graminearum, F. culmorum and F. pseudograminearium and identification of NPS2 as the producer of ferricrocin
- C. Tobiasen, Johan Aahman, Kristine Slot Ravnholt, M. Bjerrum, M. Grell, H. Giese
- BiologyCurrent Genetics
- 17 October 2006
Chemical analysis of a F. graminearum NPS2 deletion mutant showed that this gene encodes the NPS responsible for the biosynthesis of ferricrocin.
The biosynthetic pathway for aurofusarin in Fusarium graminearum reveals a close link between the naphthoquinones and naphthopyrones
Replacement of aurR1 with hygB shows that it encodes a positively acting transcription factor that is required for the full expression of PKS12, aurJ, aurF, gip1 and FG02329.1, which belong to the gene cluster, and bio‐ and chemoinformatics combined with chemical analysis of replacement mutants indicate a five‐step enzyme catalysed pathway for the biosynthesis of aurofusarin.
Leaf‐specific thionins of barley—a novel class of cell wall proteins toxic to plant‐pathogenic fungi and possibly involved in the defence mechanism of plants
A novel class of highly abundant polypeptides with antifungal activity has been detected in cell walls of barley leaves, suggesting that thionins are a naturally occurring, inducible plant protein possibly involved in the mechanism of plant defence against microbial infections.
An update to polyketide synthase and non-ribosomal synthetase genes and nomenclature in Fusarium.
The PKS4 Gene of Fusarium graminearum Is Essential for Zearalenone Production
Two aurofusarin mutants of Fusarium pseudograminearum produce elevated levels of zearalenone (ZON), one of the estrogenic mycotoxins comprising the zEARalenones, demonstrating that one polyketide can influence the expression of others.
Two Novel Classes of Enzymes Are Required for the Biosynthesis of Aurofusarin in Fusarium graminearum*
- Rasmus J. N. Frandsen, Claes Schütt, H. Giese
- BiologyThe Journal of Biological Chemistry
- 4 February 2011
Functional information is provided on two novel classes of proteins and their contribution to polyketide pigment biosynthesis in Fusarium graminearum and aurZ and aurS.