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Improving structural integrity of cryosections for immunogold labeling
It is found that the step during which sections are thawed and transferred from the cryochamber to the supporting film on an EM grid is most critical for structural preservation, and substituting a mixture of methylcellulose and sucrose for the conventional sucrose transfer medium was able to alleviate the problem of overstretching, thus improving greatly the structural integrity of thin cryosections. Expand
Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat
The results suggest that in the presence of insulin GLUT 4 recycles from the cell surface, probably via the coated pit-endosome pathway that has been characterized for cell surface receptors, and also that insulin causes the redistribution ofGLUT 4 by stimulating exocytosis from GLUT 2-containing tubulo-vesicular structures, rather than by slowing endocytotic of GLUT 3. Expand
The Human Cytomegalovirus US11 Gene Product Dislocates MHC Class I Heavy Chains from the Endoplasmic Reticulum to the Cytosol
Human cytomegalovirus (HCMV) down-regulates expression of MHC class I products by selective proteolysis by encodes an endoplasmic reticulum resident type-I transmembrane glycoprotein, which dislocates newly synthesized class I molecules from the ER to the cytosol, where they are acted upon by an N-glycanase and the proteasome. Expand
Cryosectioning and immunolabeling
Cryoimmunolabeling methods for the subcellular localization of proteins and certain lipids and the detailed procedures that have been developed and tested in practice in the laboratory during the past decades are described. Expand
Human Macrophages Accumulate HIV‐1 Particles in MHC II Compartments
The data suggest that virus particles may access the lumen of the major histocompatibility complex class II compartment by budding from the limiting membrane, thus acquiring proteins of this membrane such as class II and CD63, which could account for the particular content in lipids and proteins previously found in the membrane wrapping HIV particles. Expand
A new method of preparing gold probes for multiple-labeling cytochemistry.
  • J. Slot, H. Geuze
  • Biology, Medicine
  • European journal of cell biology
  • 1 July 1985
The usefulness of the new gold probes bound to protein A for multiple labeling in a current immunocytochemical study on receptor mediated transport of IgA in human duodenal crypt cells is demonstrated. Expand
Segregation of MHC class II molecules from MHC class I molecules in the Golgi complex for transport to lysosomal compartments
The intracellular distribution of MHC class I and class II molecules reflects the dichotomy in presentation of antigen from endogenous and exogenous origin, respectively. Expand
Vesicular Tubular Clusters between the ER and Golgi Mediate Concentration of Soluble Secretory Proteins by Exclusion from COPI-Coated Vesicles
Data suggest an important role of VTCs in soluble cargo concentration by exclusion from COPI-coated domains, as well as KDELr and rBet1, which showed a first, significant concentration in COPII- coated ER buds and vesicles and were prominently present in COPI -coated tips of V TC tubules. Expand
Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway
Cholesterol displays a highly differential distribution in the various membrane domains of the endocytic pathway of human B lymphocytes, which is almost absent from lysosomes and present in two types of multivesicular bodies. Expand
Modulators of cyclic AMP metabolism induce syncytiotrophoblast formation in vitro.
A system in which single cytotrophoblast-like cells of the human choriocarcinoma cell line undergo fusion and extensive morphological differentiation following their treatment with effectors of cyclic AMP metabolism is defined to examine the biochemical mechanism of placental cell fusion in the absence of other variables of cell differentiation. Expand