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Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3
TLDR
A cDNA library from Aspergillus niger strain NRRL‐3 enriched in sequences encoding the glucose oxidase was constructed and the deduced protein sequence was verified by partial peptide sequencing.
L-aspartate oxidase from Escherichia coli. I. Characterization of coenzyme binding and product inhibition.
TLDR
Modification of a previously published procedure allowed overexpression of the holoenzyme in an unproteolysed form and biochemical characterization of the flavoprotein L-aspartate oxidase from Escherichia coli is reported.
Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli.
TLDR
The nucleotide-sequence analysis of the gene and its flanking regions has revealed that the proteinase-K gene is composed of two exons and one 63-bp-long intron located in the proregion, and a putative promoter sequence and a capping site have been identified, suggesting that the transcription-start site is located 103-bp upstream of the ATG initiation codon.
The acid‐stable proteinase inhibitor of human mucous secretions (HUSI‐I, antileukoprotease)
The complete amino acid sequence of human antileukoprotease has been determined by direct sequencing of the inhibitory active protein isolated from seminal plasma (HUSI‐I) and by sequence analysis of
Synthesis of Apolipoprotein A‐1 in Pig Brain Microvascular Endothelial Cells
TLDR
It is shown that cultured brain microvascular endothelial cells provide an in vitro model to study the expression and function of apo A‐1 in the microvasculature of the brain.
Expression of Apolipoprotein A‐I in Porcine Brain Endothelium In Vitro
TLDR
Investigation of protein synthesis and secretion in primary cultures of porcine brain microvascular endothelial cells showed that cerebral endothelium was responsive to apo A‐I‐inducing agents, such as cholesterol, insulin, and retinoic acid, as previously shown in human hepatoma HepG2 cells.
Identification and isolation of glucose dehydrogenase genes of Bacillus megaterium M1286 and their expression in Escherichia coli.
TLDR
This work indicates the existence of at least two independent glucose dehydrogenase genes in B. megaterium M1286, which was also directly expressed in E. coli by using this gene as a hybridization probe.
Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase.
TLDR
The expression of the nadB gene under control of the inducible left promoter of the bacteriophage lambda is reported on and there is evidence for a relationship to fumarate reductase and succinate dehydrogenase of E. coli.
Complex formation between ribosomal protein S1, oligo-and polynucleotides: chain length dependence and base specificity.
In order to examine the nature of the complex formation between the ribosomal protein S1 and nucleic acids three methods were used: Inhibition of the reaction of n-ethyl[2.3 14C]-maleimide with S1 by
Codon-anticodon interaction studies with trinucleoside diphosphates containing 2-thiouridine, 4-thiouridine, 2,4-diethiouridine, or 2-thiocytidine.
Abstract 1. Analogues of U-U-U in which uridine residues were replaced by either 2-thiouridine, 4-thiouridine, 2,4-dithiouridine, or 2-thiocytidine were synthesized by enzymatic methods. 2. The
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