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Glycerol production by microbial fermentation: a review.
Improvements have been made in the glycerol production using osmotolerant yeasts on a commercial scale in China in recent years and a carrier distillation technique with a Glycerol distillation efficiency greater than 90% has been developed. Expand
Glycerol production by a novel osmotolerant yeast Candida glycerinogenes
Candida glycerinogenes, an osmotolerant yeast isolated from a natural sample in an environment of high osmotic pressure, had a modest sugar-tolerance and an extremely high glycerol productivity and was obtained by using a 30-l agitated fermentor under optimal fermentation conditions. Expand
Expression of dha Operon Required for 1,3-PD Formation in Escherichia coli and Saccharomyces cerevisiae
The results demonstrated that the recombinant S. cerevisiae strain W303-1A-BT that can produce 1,3-PD at low cost was constructed and might open a novel way to a safe and cost-efficient method for microbial production of 1, 3-PD. Expand
Construction of a Novel Expression System in Klebsiella pneumoniae and its Application for 1,3-Propanediol Production
The first reported expression system of Klebsiella pneumoniae has paved the way for improvement of 1,3-PD production and will be available and efficient for other heterologous gene expression in K. pneumoniae. Expand
Engineered Saccharomyces cerevisiae that produces 1,3‐propanediol from d‐glucose
This study aims to engineer a S. cerevisiae strain that can produce 1,3‐propanediol at low cost due to its availability being restricted owing to its expensively chemical synthesis. Expand
Construction of a novel recombinant Escherichia coli strain capable of producing 1,3–propanediol and optimization of fermentation parameters by statistical design
A mathematical model was developed and compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a significant increase in the yield of 1,3-propanediol, which makes the engineered strain have potential application in the conversion of glycerol to 1, 3-pro panediol on an industrial scale. Expand
Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis
An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE, with little increase upon Ca2+ and Mg2+ addition. Expand
budC knockout in Klebsiella pneumoniae for bioconversion from glycerol to 1,3‐propanediol
The results of the mutant fed‐batch fermentation showed that the 1,3‐PDO concentration, productivity per cell dry weight, and conversion rate showed increasing by 10%, 15%, and 11% compared with the parent strain, which implies that K. pneumoniae possesses a pathway of the 2, 3‐BD cycle as a replenishment pathway. Expand
Production of 1,3-propanediol from glycerol by engineered Escherichia coli using a novel co- expression vector
1,3-Propanediol has versatile applications in polymers, cosmetics, foods and medicines and the co-expression system, T7RNA polymerase promoter helps to consolidate the functions of glycerol dehydratase genedhaB and 1, 3-propanediol oxidoreductase gene dhaT. Expand
Construction of a flocculating yeast for fuel ethanol production
The expression vector pYX212 harboring FLO1 gene and kanMX gene was transformed into Saccharomyces. cerevisiae ZWA46. The transformant, ZWA46-F2, showed strong and stable flocculation ability duringExpand