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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, byExpand
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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specificExpand
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Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
The discovery of specific restriction endonucleases (Smith and Wilcox 1970) made possible the isolation of discrete molecular fragments of naturally occurring DNA for the first time. This capabilityExpand
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Enzymatic amplification of ?-globin genomic sequences and restriction site analysis for diagnosis of
Untreated (–) and ExoSAP-IT treated (+) PCR products were analyzed by gel electrophoresis. A variety of PCR products of different lengths may be treated with ExoSAP-IT, with no sample loss. 125 bpExpand
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Genome-wide association study and meta-analysis find that over 40 loci affect risk of type 1 diabetes
Type 1 diabetes (T1D) is a common autoimmune disorder that arises from the action of multiple genetic and environmental risk factors. We report the findings of a genome-wide association study of T1D,Expand
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Polymerase chain reaction
  • H. Erlich
  • Biology, Medicine
  • Journal of Clinical Immunology
  • 1 November 1989
SummaryThe PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragmentExpand
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Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a singleExpand
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Recent advances in the polymerase chain reaction
The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carriedExpand
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Risk of celiac disease autoimmunity and timing of gluten introduction in the diet of infants at increased risk of disease.
CONTEXT While gluten ingestion is responsible for the signs and symptoms of celiac disease, it is not known what factors are associated with initial appearance of the disease. OBJECTIVE To examineExpand
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PCR Technology: Principles and Applications for DNA Amplification
This introductory guide covers the basic methodology, research applications, and medical applications of PCR technology, emphasizing the practical aspects of the technique. Every chapter is writtenExpand
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