Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific…
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
- K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich
- BiologyCold Spring Harbor Symposia on Quantitative…
An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap.
Polymerase chain reaction
- H. Erlich
- BiologyJournal of Clinical Immunology
In addition to its many applications in basic molecular biological research, PCR promises to play a critical role in the identification of medically important sequences as well as an important diagnostic one in their detection.
Enzymatic amplification of ?-globin genomic sequences and restriction site analysis for diagnosis of
ExoSAP-IT offers quick, one step PCR clean-up with 100% recovery of both short and long PCR products, and is completely scaleable for manual or automated use.
Genome-wide association study and meta-analysis find that over 40 loci affect risk of type 1 diabetes
A genome-wide association study and meta-analysis find that over 40 loci affect risk of type 1 diabetes, with convincing support for 19 additional T1D-associated loci5–13, all with allelic odds ratios (ORs) of less than 1.3.
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
This work has studied the allelic diversity at the HLA-DQA locus and its association with the serologically defined Hla-DR and -DQ types and revealed eight alleles and three additional haplotypes.
Influence of combinations of human major histocompatibility complex genes on the course of HIV–1 infection
An HLA profile was produced that predicted time from HIV–1 infection to the onset of AIDS and support current theory about control of antigen processing by HLA genes and have implications for immunopathogenesis of HIV-1 and other infections.
PCR Technology: Principles and Applications for DNA Amplification
- H. Erlich
- 1 October 1989
This introductory guide covers the basic methodology, research applications, and medical applications of PCR technology, emphasizing the practical aspects of the technique. Every chapter is written…
HLA DR-DQ Haplotypes and Genotypes and Type 1 Diabetes Risk
Data indicate that the risk associated with specific HLA haplotypes can be influenced by the genotype context and that the trans-complementing heterodimer encoded by DQA1*0501 and DQB1*0302 confers very high risk.