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Disclosure of the mycobacterial outer membrane: Cryo-electron tomography and vitreous sections reveal the lipid bilayer structure
Cryo-electron tomography of unperturbed or detergent-treated cells of Mycobacterium smegmatis embedded in vitreous ice now reveals the native organization of the cell envelope and its delineation into several distinct layers, crucial for the investigation and understanding of transport processes across the mycobacterial cell wall. Expand
Structural research on surface layers: a focus on stability, surface layer homology domains, and surface layer-cell wall interactions.
The structural requirements for the extraordinary stability of many S- layer proteins, the structural and functional aspects of the S-layer homology domain found in S-layers, extracellular enzymes and related functional proteins, and outer membrane proteins,and the molecular interactions of S- Layer proteins with other cell wall components are discussed. Expand
Are S-layers exoskeletons? The basic function of protein surface layers revisited.
  • H. Engelhardt
  • Biology, Medicine
  • Journal of structural biology
  • 1 November 2007
The synergistic effects in cell envelope assemblies, the hypothetical role of S-layers for cell shape formation, and the existence of a common function are discussed in view of new insights. Expand
Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes.
The structure of 20 S particles, consisting of NSF, SNAPs, and SNARE complexes, was analyzed by electron microscopy and fluorescence resonance energy transfer to define the contribution of each component to the 20 S particle structure. Expand
MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis
Results show that MspA is the main general diffusion pathway for hydrophilic molecules in M. smegmatis and was only partially replaced by fewer porins in the cell wall of the ΔmspA mutant, the lowest value reported so far for bacteria. Expand
Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis.
The three-dimensional structure of the Acetogenium kivui surface layer (S-layer) has been determined to a resolution of 1.7 nm by electron crystallographic techniques and is the first example of a domain conserved in several S-layer proteins. Expand
Tom40, the Pore-Forming Component of the Protein-Conducting Tom Channel in the Outer Membrane of Mitochondria
Tom40 is the core element of the TOM translocase; it forms the protein-conducting channel in an oligomeric assembly and interacts specifically with Mitochondrial presequence peptides to reconstituted into planar lipid membranes. Expand
Tetrabrachion: a filamentous archaebacterial surface protein assembly of unusual structure and extreme stability.
The surface (S-) layer of the hyperthermophilic archaebacterium Staphylothermus marinus was isolated, dissected into separate domains by chemical and proteolytic methods, and analyzed by spectroscopic, electron microscopic and biochemical techniques to represent one of the most stable proteases characterized to date. Expand
The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum
The reverse transcription–polymerase chain reaction (RT–PCR) analysis revealed that the genes flaE to flaK are transcribed into one polycistronic message, indicating that the flaI gene product is involved in the biosynthesis, transport or assembly of flagella in H. salinarum. Expand
A Tetrameric Porin Limits the Cell Wall Permeability ofMycobacterium smegmatis *
The length of MspA is sufficient to span the outer membrane and contributes in combination with the tapering end of the pore and the low number of pores to the low permeability of the cell wall of M. smegmatis for hydrophilic compounds. Expand