• Publications
  • Influence
Large-scale mapping of human protein–protein interactions by mass spectrometry
Mapping protein–protein interactions is an invaluable tool for understanding protein function. Here, we report the first large‐scale study of protein–protein interactions in human cells using a massExpand
  • 876
  • 37
  • PDF
Crystal structure of the lytic transglycosylase from bacteriophage lambda in complex with hexa-N-acetylchitohexaose.
The three-dimensional structure of the lytic transglycosylase from bacteriophage lambda, also known as bacteriophage lambda lysozyme, complexed to the hexasaccharide inhibitor,Expand
  • 44
  • 5
Substrate and Metal Complexes of 3-Deoxy-d-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Å Resolution
3-Deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacteriumAquifex aeolicus differs from its Escherichia coli counterpart in the requirement of a divalent metalExpand
  • 60
  • 4
Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation
ABSTRACT The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefoldExpand
  • 135
  • 3
Functional and biochemical characterization of a recombinant 3-Deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus.
The kdsA gene from the hyperthermophilic bacterium Aquifex aeolicus was cloned into a vector for expression in Escherichia coli and the kdsA gene product, 3-deoxy-d-manno-octulosonic acid 8-phosphateExpand
  • 27
  • 2
Design and Analysis of Quantitative Differential Proteomics Investigations Using LC-MS Technology
Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is becoming an increasingly important tool in characterizing the abundance of proteins in biological samples of various types andExpand
  • 21
  • 2
  • PDF
A Metal Bridge between Two Enzyme Families
The enzymes 3-deoxy-d-manno-octulosonic acid-8-phosphate synthase (KDO8PS) and 3-deoxy-d-arabino-heptulosonic acid-7-phosphate synthase (DAHPS) catalyze analogous condensation reactions betweenExpand
  • 44
  • 1
  • PDF
The proteomic reactor: a microfluidic device for processing minute amounts of protein prior to mass spectrometry analysis.
Gel-free proteomics has emerged as a complement to conventional gel-based proteomics. Gel-free approaches focus on peptide or protein fractionation, but they do not address the efficiency of proteinExpand
  • 71
  • 1
Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis.
We have determined the crystal structures of the metalloenzyme 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PEP) and riboseExpand
  • 39
  • 1
Incorporation of trifluoromethionine into a phage lysozyme: implications and a new marker for use in protein 19F NMR.
Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesisExpand
  • 73
  • 1