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Large-scale mapping of human protein–protein interactions by mass spectrometry
Mapping protein–protein interactions is an invaluable tool for understanding protein function. Here, we report the first large‐scale study of protein–protein interactions in human cells using a mass… Expand
Crystal structure of the lytic transglycosylase from bacteriophage lambda in complex with hexa-N-acetylchitohexaose.
The three-dimensional structure of the lytic transglycosylase from bacteriophage lambda, also known as bacteriophage lambda lysozyme, complexed to the hexasaccharide inhibitor,… Expand
Substrate and Metal Complexes of 3-Deoxy-d-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Å Resolution
- H. Duewel, S. Radaev, J. Wang, R. Woodard, D. Gatti
- Chemistry, Medicine
- The Journal of Biological Chemistry
- 16 March 2001
3-Deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacteriumAquifex aeolicus differs from its Escherichia coli counterpart in the requirement of a divalent metal… Expand
Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation
- Keith Q. Tanis, D. Veach, H. Duewel, W. Bornmann, A. J. Koleske
- Medicine, Biology
- Molecular and Cellular Biology
- 1 June 2003
ABSTRACT The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefold… Expand
Functional and biochemical characterization of a recombinant 3-Deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus.
- H. Duewel, G. Y. Sheflyan, R. Woodard
- Biology, Medicine
- Biochemical and biophysical research…
- 24 September 1999
The kdsA gene from the hyperthermophilic bacterium Aquifex aeolicus was cloned into a vector for expression in Escherichia coli and the kdsA gene product, 3-deoxy-d-manno-octulosonic acid 8-phosphate… Expand
Design and Analysis of Quantitative Differential Proteomics Investigations Using LC-MS Technology
- Yury V. Bukhman, M. Dharsee, +8 authors N. F. Ng
- Computer Science, Biology
- J. Bioinform. Comput. Biol.
- 1 February 2008
Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is becoming an increasingly important tool in characterizing the abundance of proteins in biological samples of various types and… Expand
A Metal Bridge between Two Enzyme Families
The enzymes 3-deoxy-d-manno-octulosonic acid-8-phosphate synthase (KDO8PS) and 3-deoxy-d-arabino-heptulosonic acid-7-phosphate synthase (DAHPS) catalyze analogous condensation reactions between… Expand
The proteomic reactor: a microfluidic device for processing minute amounts of protein prior to mass spectrometry analysis.
- M. Ethier, Weimin Hou, H. Duewel, D. Figeys
- Chemistry, Medicine
- Journal of proteome research
- 22 August 2006
Gel-free proteomics has emerged as a complement to conventional gel-based proteomics. Gel-free approaches focus on peptide or protein fractionation, but they do not address the efficiency of protein… Expand
Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis.
We have determined the crystal structures of the metalloenzyme 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PEP) and ribose… Expand
Incorporation of trifluoromethionine into a phage lysozyme: implications and a new marker for use in protein 19F NMR.
Much interest is currently focused on understanding the detailed contribution that particular amino acid residues make in protein structure and function. Although the use of site-directed mutagenesis… Expand