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Isolation, characterization, and mapping to chromosome 19 of the human apolipoprotein E gene.
The apo-E gene was mapped in the human genome to chromosome 19 through the use of DNA probes and human-rodent somatic cell hybrids and the DNA sequence was compared with that of a near full length cDNA clone pE-368 and revealed three introns. Expand
Cell type-specific expression of the human apoB gene is controlled by two cis-acting regulatory regions.
Using the gel mobility shift assay and the DNase I footprinting technique, it is demonstrated that DNA binding proteins from HepG2 and mouse liver nuclear extracts interact with the crucial positive region located between -86 and -70, suggesting that these genes may share some transcriptional regulatory components. Expand
An Upstream Element Containing an ETS Binding Site Is Crucial for Transcription of the Human Presenilin-1 Gene*
Transfection and footprinting assays correlate to suggest that Sp1 transcription factor(s) bind at several sites upstream and downstream from the initiation site and activate the transcription of the PS1 promoter, suggesting that protein-protein interactions between factors recognizing downstream and upstream sequences are involved. Expand
Overexpression of human histone methylase MLL1 upon exposure to a food contaminant mycotoxin, deoxynivalenol
MLL1 gene expression is sensitive to toxic stress and Sp1 plays crucial roles in the stress‐induced upregulation of MLL1. Expand
Changes in carnitine palmitoyltransferase-I mRNA abundance produced by hyperthyroidism and hypothyroidism parallel changes in activity.
To study the regulation of carnitine palmitoyltransferase-I by thyroid hormone, a cDNA was obtained by PCR amplification of DNA obtained by reverse transcription of rat liver RNA and it was suggested that CPT-I is regulated at the transcriptional level by thyroid hormones. Expand
The human apolipoprotein C-II gene sequence contains a novel chromosome 19-specific minisatellite in its third intron.
The human apolipoprotein C-II gene was sequenced and found to contain four exons and three introns, with a major transcription initiation site located 26 base pairs downstream from a TATA sequenceExpand
Regulation of Transcription of the Human Presenilin-1 Gene by Ets Transcription Factors and the p53 Protooncogene*
It is shown here that p53 also suppresses the transcription of a presenilin-1 promoter-chloramphenicol acetyltransferase reporter synthetic gene in transient infection assays in neuroblastoma (SK-N-SH) and hepatoma (HepG2) cell lines. Expand
Ets transcription factors ER81 and Elk1 regulate the transcription of the human presenilin 1 gene promoter.
It is shown that in vitro translated ER81 indeed binds specifically to the -10 region of the PS1 promoter and that ER81 activates by two- to threefold the basal transcription of a presenilin-1 promoter-chloramphenicol acetyltransferase reporter synthetic gene (-119, +178)PS1CAT in transient infection assays in neuroblastoma cells (SK-N-SH). Expand
Membrane Topology of Human Presenilin-1 in SK-N-SH Cells Determined by Fluorescence Correlation Spectroscopy and Fluorescent Energy Transfer
Interaction between YFP–PS1 and PS1–CFP confirming the presence of a dimeric or multimeric form of PS1 in SK-N-SH cells is suggested and it is concluded that both COOH-terminal and NH2-terminals of human PS1 may also be oriented on the cytosolic side of ER membrane. Expand
Protein synthesis in rabbit reticulocytes. Purification and characterization of a double-stranded RNA-dependent protein synthesis inhibitor from reticulocyte lysates.
The results are similar to those reported previously for the heme-regulated eIF-2 kinase and suggest that dsI, like the he me- regulated e IF-2 Kinase, is inactive in some step(s) of Met-tRNAf. Expand