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Acetylene as a suicide substrate and active site probe for methane monooxygenase from Methylococcus capsulatus (Bath)
Acetylene was shown to be an inhibitor of cell-free methane monooxygenase (MMO) activity in Methylococcus capsulatus (Bath). Inhibition was demonstrated for both the soluble and particulate forms ofExpand
Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: Studies in batch and continuous cultures
SummaryThe intracellular location of methane mono-oxygenase (MMO) activity in the methanotroph Methylococcus capsulatus (Bath) has been shown to depend primarily on the availability of copper. MMOExpand
The membrane-associated form of methane mono-oxygenase from Methylococcus capsulatus (Bath) is a copper/iron protein.
The essential role of copper in enzyme catalysis is verified, the implausibility of copper existing as a trinuclear cluster is indicated and the presence of a tightly bound mononuclear Fe(3+) ion in an octahedral environment that may well be exchange-coupled to another paramagnetic species is demonstrated. Expand
The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.
Methane mono-oxygenase of Methylococcus capsulatus (Bath) catalyses the oxidation of various substituted methane derivatives including methanol and, in some of its catalytic properties, apparently resembles the analogous enzyme from Methylomonas methanica but differs from those found in Methylosinus trichosporium and M methylomonas albus. Expand
The effect of copper ions on membrane content and methane monooxygenase activity in methanol-grown cells of Methylococcus capsulatus (Bath)
SUMMARY: Methylococcus capsulatus (Bath) was grown in continuous culture with methanol (1·0%, v/v) as sole carbon and energy source. Cells grown on methanol exhibited differences in methaneExpand
The Methylotrophic Bacteria
Three groups of microbes are considered here: the methane utilizers, the methanol utilizers, and the carbon monoxide utilizers. The methane utilizers (methanotrophs) appear to be composed ofExpand
The Leeuwenhoek Lecture 2000 The natural and unnatural history of methane-oxidizing bacteria
  • H. Dalton
  • Medicine, Biology
  • Philosophical Transactions of the Royal Society B…
  • 29 June 2005
Understanding of how bacteria are capable of effecting one of the most difficult reactions in chemistry—namely, the controlled oxidation of methane to methanol—has been made possible by the isolation, in pure form, of the enzyme components. Expand
Enantioselective bacterial biotransformation routes to cis-diol metabolites of monosubstituted benzenes, naphthalene and benzocycloalkenes of either absolute configuration
Enzyme-catalysed kinetic resolution and asymmetric dihydroxylation routes to enantiopure cis-diol metabolites of arenes and benzocycloalkenes of either absolute configuration have been developedExpand
The methane monooxygenase gene cluster of Methylococcus capsulatus (Bath).
The complete nucleotide sequence of mmoX, the gene encoding the alpha-subunit of component A of MMO component A which is found to be 5' to mmoY and mmoZ, is reported and it is reported that this gene is significantly homologous with spinach ferredoxin. Expand
Nitrogen Fixation in Obligate Methanotrophs
A number of representative species of obligate methane-oxidizing bacteria were surveyed for their ability to fix N2 by growth experiments and the acetylene reduction test and only type II organisms and the type X methanotroph Methylococcus capsulatus (Bath) grew well in nitrogen-free liquid medium and were capable of active acetylene Reduction. Expand