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Circular DNA elements of chromosomal origin are common in healthy human somatic tissue
It is shown that normal human cells generate large extrachromosomal circular DNAs (eccDNAs), most likely the products of excised DNA, that can be transcriptionally active and, thus, may have phenotypic consequences.
Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells.
  • H. D. Møller
  • Medicine, Chemistry
    Methods in molecular biology
  • 2020
The Circle-Seq protocol is described, applicable for physical enrichment of eccDNAs of a broad size range, combined with sequence confirmation of circular structures, which could be detected at nucleotide resolution in a million human muscle nuclei by this method.
Extrachromosomal circular DNA is common in yeast
It is proposed that eccDNAs may be precursors to the copy number variation in eukaryotic genomes characteristic of both the evolutionary process and cancer progression, and that they might contribute substantially to genetic variation and evolution.
Regulation of apoptosis and autophagy in mouse and human skeletal muscle with aging and lifelong exercise training
There was a divergent regulation of autophagy and apoptosis in mouse muscle with aging and lifelong exercise training, whereas healthy aged human skeletal muscle seemed rather robust to changes in apoptosis, autophagic and mitophagy markers compared with mouse muscle at the investigated age.
CRISPR-C: circularization of genes and chromosome by CRISPR in human cells
This study creates a new territory forCRISPR gene editing and highlights CRISPR-C as a useful tool for studying the cellular impact, persistence and function of eccDNAs.
Lifelong physical activity is associated with promoter hypomethylation of genes involved in metabolism, myogenesis, contractile properties and oxidative stress resistance in aged human skeletal muscle
The results suggest that lifelong physical activity is associated with DNA methylation patterns that potentially allow for increased insulin sensitivity and a higher expression of genes in energy metabolism, myogenesis, contractile properties and oxidative stress resistance in skeletal muscle of aged individuals.
Amino Acid Transporter Genes Are Essential for FLO11-Dependent and FLO11-Independent Biofilm Formation and Invasive Growth in Saccharomyces cerevisiae
It is discovered that the budding yeast Saccharomyces cerevisiae CEN.PK evolved invasive growth ability under prolonged nitrogen limitation and the amino acid transporter gene GAP1 was found to influence invasive growth through FLO11 as well as other FLO genes.
Formation of Extrachromosomal Circular DNA from Long Terminal Repeats of Retrotransposons in Saccharomyces cerevisiae
Recombination junctions are detected that are consistent with yeast Ty eccDNAs being generated through recombination events within the genome, opening the possibility that retrotransposable elements could move around in the genome without an RNA intermediate directly through DNA circularization.
A model for generating several adaptive phenotypes from a single genetic event
It is suggested that a population can rapidly adapt to multiple environmental changes if individual members stochastically flip a hub-switch that controls a set of adaptive phenotypes in a single event.
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells.
The Circle-Seq method has broad applicability for genome-scale screening for eccDNA in eukaryotes as well as for detecting specific eccDNA types.