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The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence.
The fungus Rhizopus oryzae synthesizes an extracellular lipase precursor bearing N-terminal pre- and pro-sequences. Our studies in Escherichia coli and using recombinant lipase in vitro indicate thatExpand
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Ion Chromatographie Separation and Spectrophotometric Determination of U(IV) and U(VI)
An ion Chromatographie method is described for the simultaneous determination of U(IV) and U(VI) species in mixtures of uranium oxides and fluorides. The Separation of the uranium species wasExpand
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Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants.
Both an active enzyme conformation and stabilization of tetrahedral transition states are essential for the catalysis of ester bond hydrolysis by lipases. X-ray structural data and results fromExpand
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On-line preconcentration of silver on activated alumina and determination in borehole water by flow injection atomic absorption spectrophotometry
SummaryA micro column of activated alumina in the basic form was used in conjunction with flame atomic absorption for the preconcentration and determination of silver in borehole water. The silverExpand
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Vanadium speciation by ion chromatography
An ion-chromatographic method, using a carbonate-buffered (1,2-cyclohexylenedinitrilo)tetraacetic acid (CDTA) eluant, is described for the simultaneous determination of vanadium(IV) and vanadium(V).Expand
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