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Functional profiling of the Saccharomyces cerevisiae genome
TLDR
It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
TLDR
A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.
The Genetic Landscape of a Cell
TLDR
A network based on genetic interaction profiles reveals a functional map of the cell in which genes of similar biological processes cluster together in coherent subsets, and highly correlated profiles delineate specific pathways to define gene function.
Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
TLDR
A method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants is developed, which should produce a global map of gene function.
Life with 6000 Genes
TLDR
The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration and provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history.
Global Mapping of the Yeast Genetic Interaction Network
TLDR
Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.
An in Vivo Map of the Yeast Protein Interactome
TLDR
A genome-wide in vivo screen for protein-protein interactions in Saccharomyces cerevisiae by means of a protein-fragment complementation assay (PCA) identified 2770 interactions among 1124 endogenously expressed proteins, revealing a previously unexplored subspace of the yeast protein interactome.
Saccharomyces cerevisiae Mid2p Is a Potential Cell Wall Stress Sensor and Upstream Activator of thePKC1-MPK1 Cell Integrity Pathway
TLDR
Data are consistent with a role for Mid2p in sensing cell wall stress and in activation of a response that includes both increased chitin synthesis and the Mpk1p mitogen-activated protein kinase cell integrity pathway.
Cell Wall Assembly in Saccharomyces cerevisiae
  • G. Lesage, H. Bussey
  • Biology, Engineering
    Microbiology and Molecular Biology Reviews
  • 1 June 2006
TLDR
An area where functional genomics offers new insights into the integration of cell wall growth and morphogenesis with a polarized secretory system that is under cell cycle and cell type program controls is reviewed.
Exploring genetic interactions and networks with yeast
TLDR
A comparative understanding of genetic-interaction networks promises insights into some long-standing genetic problems, such as the nature of quantitative traits and the basis of complex inherited disease.
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