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Purification, characterization, and crystallization of monoamine oxidase from Escherichia coli K-12.
The gene for monoamine oxidase (MAO) was cloned from an Escherichia coli genomic library and MAO was overproduced in the periplasmic space. The enzyme was purified to homogeneity by preparation of aExpand
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Lipophilization of lysozyme by short and middle chain fatty acids.
Hen egg white lysozyme was lipophilized with short and middle chain saturated fatty acids (caproic, capric, or myristic acid). The yield, bactericidal properties, and structural properties ofExpand
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Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms
The aim of this study was to investigate the effects of tea catechin epigallocatechin gallate (EGCg) on established biofilms and biofilm formation by Porphyromonas gingivalis, a major pathogen ofExpand
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Bactericidal action of egg yolk phosvitin against Escherichia coliunder thermal stress.
Chicken egg yolk phosvitin showed a remarkable antibacterial effect against Escherichia coli under thermal stress at 50 degrees C. E. coli cells (10(6)/mL) completely disappeared in 1 mL of L-brothExpand
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Effects of Er:YAG laser irradiation on biofilm-forming bacteria associated with endodontic pathogens in vitro.
With the development of dental laser delivery systems that can enter into the root canals, it is possible to use Er:YAG lasers to remove the residual biofilm associated with infected root canals. WeExpand
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Effects of calnexin deletion in Saccharomyces cerevisiae on the secretion of glycosylated lysozymes.
Disruption of the calnexin gene in Saccharomyces cerevisiae did not lead to gross effects on the levels of cell growth and secretion of wild-type hen egg white lysozymes (HEWL). To investigate theExpand
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Characterization of recombinant amyloidogenic chicken cystatin mutant I66Q expressed in yeast.
Amyloidogenic chicken cystatin mutant I66Q (cC I66Q) was successfully secreted by yeasts Pichia pastoris and Saccharomyces cerevisiae. The soluble monomer and dimer forms of amyloidogenic cC I66QExpand
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Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli
Abstract We found that the structural gene for monoamine oxidase was located at 30.9 min on the Escherichia coli chromosome. Deletion analysis showed that two amine oxidase genes are located in thisExpand
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Characterization of autoinducer 2 signal in Eikenella corrodens and its role in biofilm formation.
Quorum sensing (QS) is a process by which bacteria communicate using secreted chemical signaling molecules called autoinducers (AIs). By this process, many bacterial species modulate the expressionExpand
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Role of amyloid type cross beta-structure in the formation of soluble aggregate and gel in heat-induced ovalbumin.
The heat-induced denaturation curve of ovalbumin followed by the ellipticity at 222 nm in circular dichroism spectra was consistent with that monitored by fluorescence with thioflavin T, which is anExpand
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