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Tuning genetic control through promoter engineering.
Gene function is typically evaluated by sampling the continuum of gene expression at only a few discrete points corresponding to gene knockout or overexpression. We argue that this characterizationExpand
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Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show theExpand
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Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production.
Economic feasibility of biosynthetic fuel and chemical production hinges upon harnessing metabolism to achieve high titre and yield. Here we report a thorough genotypic and phenotypic optimization ofExpand
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Metabolic engineering of muconic acid production in Saccharomyces cerevisiae.
The dicarboxylic acid muconic acid has garnered significant interest due to its potential use as a platform chemical for the production of several valuable consumer bio-plastics including nylon-6,6Expand
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Global transcription machinery engineering: a new approach for improving cellular phenotype.
It is now generally accepted that most cellular phenotypes are affected by many genes. As a result, engineering a desired phenotype would be facilitated enormously by simultaneous multiple geneExpand
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Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets
Identification of genes that affect the product accumulation phenotype of recombinant strains is an important problem in industrial strain construction and a central tenet of metabolic engineering.Expand
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Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli.
The identification of genetic targets that are effective in bringing about a desired phenotype change is still an open problem. While random gene knockouts have yielded improved strains in certainExpand
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Rewiring yeast sugar transporter preference through modifying a conserved protein motif
Significance The quest for an optimal xylose pathway in yeast is of utmost importance along the way to realizing the potential of lignocellulosic biomass conversion into fuels and chemicals. AnExpand
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Promoter engineering: Recent advances in controlling transcription at the most fundamental level
Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically, precise control of key pathway enzymes (heterologous or native) can help maximize product formation.Expand
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Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach†
ABSTRACT The development of strong and tunable promoter elements is necessary to enable metabolic and pathway engineering applications for any host organism. Here, we have expanded and generalized aExpand
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