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The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)
Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3× draft genome sequence of ‘SunUp’ papaya, theExpand
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Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome.
The bacteriophage P1 Cre-lox site-specific recombination system has been used to integrate DNA specifically at lox sites previously placed in the tobacco genome. As integrated molecules flanked byExpand
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Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced.
In an effort to control the variability of transgene expression in plants, we used Cre-lox mediated recombination to insert a gus reporter gene precisely and reproducibly into different target loci.Expand
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Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells
Transgenic sugarcane (Saccharum officinarum L.) lines were created to express altered invertase isoform activity to elucidate the role(s) of invertase in the sucrose accumulation process. A sugarcaneExpand
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Production of biologically active GM-CSF in sugarcane: a secure biofactory
Over 300 transgenic sugarcane plants representing approx. 200 independent lines producing the human cytokine granulocyte macrophage colony stimulating factor (GM-CSF) were analyzed for recombinantExpand
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The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities.
The Sugarcane yellow leaf virus (SCYLV) P0, a member of the highly heterologous proteins of poleroviruses, is a suppressor of posttranscriptional gene silencing (PTGS) and has additional activitiesExpand
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PCR amplification from a homolog of the bE mating-type gene as a sensitive assay for the presence of Ustilago scitaminea DNA
Ustilago scitaminea, the causal agent of sugarcane (Saccharum spp.) smut, has a bipolar mating system in that the haploid stage of the life cycle has sporidia of two mating types (designated plus andExpand
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Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants.
GUS (uidA) reporter gene expression for two sugarcane polyubiquitin promoters, ubi4 and ubi9, was compared to expression from the maize Ubi-1 promoter in stable transgenic rice (only ubi9) andExpand
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Molecular and phenotypic characterization of Als1 and Als2 mutations conferring tolerance to acetolactate synthase herbicides in soybean
BACKGROUND Sulfonylurea (SU) herbicides are effective because they inhibit acetolactate synthase (ALS), a key enzyme in branched-chain amino acid synthesis required for plant growth. A soybean lineExpand
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Systemic acquired resistance induced by BTH in papaya
Systemic acquired resistance (SAR) in Carica papaya L. is induced by benzothiadiazole (BTH). The response is manifested by increased tolerance to infection by the virulent pathogen PhytophthoraExpand
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