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Novel reference molecules for quantitation of genetically modified maize and soybean.
The results show that the method involving the use of these plasmids as RMs is reliable and practical for quantitation of GM maize and GM soy.
Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays).
Rec recombinant DNAs introduced into the seven lines of GM maize, such as Event 176, Bt11, T25, MON810, GA21, DLL25, and MON802, are sequenced and it is concluded that this method is useful for fast and easy screening of GM crops including unauthorized ones.
A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize.
Improved a multiplex PCR method described in the previous report in order to distinguish the five lines of genetically modified (GM) maize, and re-designed new primer pairs to increase the specificity of PCR to distinguishFive lines of GM maize by multipleX PCR.
Apple procyanidin oligomers absorption in rats after oral administration: analysis of procyanidins in plasma using the porter method and high-performance liquid chromatography/tandem mass
The results suggest that ACTs are absorbed and directly involved in physiological functions in the rats and the limits of the absorption of apple procyanidins in the polymerization degree are investigated.
A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods
A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed and would be particularly useful in the detection of hidden meat mince in processed foods, which would verify food labeling and gain consumers’ trust.
Estimation of daily aluminum intake in Japan based on food consumption inspection results: impact of food additives
The daily dietary Al intake from processed foods was much larger than that from unprocessed foods, and only the highest consumer Al exposure value of the young children group exceeded the PTWI.
Japan food allergen labeling regulation--history and evaluation.
The selection of the specific allergenic ingredients by the Japanese government, the implementation of regulatory action levels and the detection methods to support them, and the assessment of the effectiveness of this approach are reviewed.
Indicated detection of two unapproved transgenic rice lines contaminating vermicelli products.
The Bacillus thuringiensis (Bt) rice line, which has a construct similar to the GM Shanyou 63 line, was detected in some vermicelli products by identification of the junction region sequence between rice Act1 promoter and the Cry1Ac gene.
Validation procedures for quantitative food allergen ELISA methods: community guidance and best practices.
This document provides supplemental guidance on specifications for the development and implementation of studies to validate the performance characteristics of quantitative ELISA methods for the determination of food allergens, and certain criteria included in the guidance are allergen-specific.
Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up.
A rapid and simple method using a multifunctional column, which contains lipophilic and charged active sites, was developed to analyse aflatoxins B1, B2, G1 and G2 in various spices, such as red