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Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery
The different uptake pathways that are involved in nonviral gene delivery from a gene delivery point of view are reviewed and available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationsic polymer- DNA complexes (polyplexes) is summarized. Expand
The RNA sensor RIG-I dually functions as an innate sensor and direct antiviral factor for hepatitis B virus.
Type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. Expand
Transport Activity of Human MRP3 Expressed in Sf9 Cells: Comparative Studies with Rat MRP3
- H. Akita, Hiroshi Suzuki, T. Hirohashi, H. Takikawa, Y. Sugiyama
- Biology, Medicine
- Pharmaceutical Research
The substrate specificity of h MRP3 resembles that of rMRP3 although differences were observed, particularly in bile acid transport, and the inhibitory effect of taurocholate and glycocholate on the transport of E217βG was much more potent in rMRp3 compared to hMRP 3. Expand
Inhibition of Bile Acid Transport across Na+/Taurocholate Cotransporting Polypeptide (SLC10A1) and Bile Salt Export Pump (ABCB 11)-Coexpressing LLC-PK1 Cells by Cholestasis-Inducing Drugs
- S. Mita, Hiroshi Suzuki, +4 authors Y. Sugiyama
- Chemistry, Medicine
- Drug Metabolism and Disposition
- 1 September 2006
The study suggests that coexpressing cells can provide a useful system for the identification of inhibitors of these two transport systems, including potential drug candidates. Expand
A multifunctional envelope type nano device (MEND) for gene delivery to tumours based on the EPR effect: a strategy for overcoming the PEG dilemma.
The development and applications of MEND are described, and strategies for overcoming the PEG dilemma are discussed, based on the manipulation of intracellular trafficking of cellular uptake and endosomal release using functional devices such as specific ligands, cleavable PEG systems andendosomal fusogenic/disruptic peptides. Expand
Characterization of bile acid transport mediated by multidrug resistance associated protein 2 and bile salt export pump.
- H. Akita, H. Suzuki, +4 authors Y. Sugiyama
- Biology, Medicine
- Biochimica et biophysica acta
- 9 March 2001
In membrane vesicles where both Mrp2 and Bsep are co-expressed, preincubation with the sulfated bile acids potentiated their inhibitory effect on BseP-mediated TC transport, and these results can be accounted for by assuming that the sulfate bile acid trans-inhibit the BSEp-mediated transport of TC. Expand
Sinusoidal Efflux of Taurocholate Is Enhanced in Mrp2-Deficient Rat Liver
Enhanced sinusoidal efflux of TC in EHBR may be related to the altered disposition of bile acids in the mutant rats, and may be accounted for, at least partially, by the increased expression of Mrp3. Expand
Development of a novel systemic gene delivery system for cancer therapy with a tumor-specific cleavable PEG-lipid
In vivo studies revealed that the PPD was potent in stabilizing MEND in the systemic circulation and facilitating tumor accumulation, and MEND modified with PPD is a promising device, which has the potential to make in vivo cancer gene therapy achievable. Expand
Quantitative comparison of intracellular trafficking and nuclear transcription between adenoviral and lipoplex systems.
- S. Hama, H. Akita, R. Ito, H. Mizuguchi, T. Hayakawa, H. Harashima
- Biology, Medicine
- Molecular therapy : the journal of the American…
- 1 April 2006
A systematic and quantitative comparison of the cellular uptake and subsequent intracellular distribution of exogenous DNA transfected by viral and nonviral vectors in living cells is described, using a combination of TaqMan PCR and a recently developed confocal image-assisted three-dimensionally integrated quantification method. Expand
Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex
- S. Hama, H. Akita, S. Iida, H. Mizuguchi, H. Harashima
- Medicine, Biology
- Nucleic acids research
- 7 February 2007
An improvement in nuclear decondensation and the diminution of the interaction between vector and mRNA is essential for the development of new generations of non-viral vectors. Expand