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Molecular structure of the acyl-enzyme intermediate in β-lactam hydrolysis at 1.7 Å resolution
The X-ray crystal structure of the molecular complex of penicillin G with a deacylation-defective mutant of the RTEM-1 β-lactamase from Escherichia coli shows how these antibiotics are recognized and…
Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker.
- H. Adachi, T. Hasebe, K. Yoshinaga, T. Ohta, K. Sutoh
- BiologyBiochemical and biophysical research…
- 30 December 1994
An improved REMI (restriction enzyme-mediated integration) system for generating mutant Dictyostelium cells quickly and efficiently for systematic screening of cytokinesis mutants and revealed that a single copy of the tag DNA was integrated into each genome.
Epigenetic Inactivation of Wnt Inhibitory Factor-1 Plays an Important Role in Bladder Cancer through Aberrant Canonical Wnt/β-Catenin Signaling Pathway
This is the first report showing that CpG hypermethylation of the Wif-1 promoter is a frequent event in bladder tumor and may contribute to pathogenesis of bladder cancer through aberrant canonical Wnt/β-catenin signaling pathway.
The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide
The effect of the rodA(Sui) mutation is to indirectly increase the availability of tripeptide side chains, which are used preferentially by PBP3 as acceptors in transpeptidation.
Dictyostelium IQGAP-related Protein Specifically Involved in the Completion of Cytokinesis
- H. Adachi, Yasuhiro Takahashi, T. Hasebe, M. Shirouzu, S. Yokoyama, K. Sutoh
- BiologyThe Journal of cell biology
- 19 May 1997
Results indicate that the GAPA protein is specifically involved in the completion of cytokinesis, indicating that IQGAPs are putative effectors for Rac and CDC42, members of the Rho family of GTPases, and participate in reorganization of the actin cytoskeleton.
Site-directed mutants, at position 166, of RTEM-1 beta-lactamase that form a stable acyl-enzyme intermediate with penicillin.
A Novel Endoplasmic Reticulum Membrane Protein Rcr1 Regulates Chitin Deposition in the Cell Wall of Saccharomyces cerevisiae*
The results show that RCR1 regulates the chitin deposition and add firm genetic and biochemical evidences that the primary target of Congo red is chitIn in S. cerevisiae.
Immunoisolaton of the Yeast Golgi Subcompartments and Characterization of a Novel Membrane Protein, Svp26, Discovered in the Sed5-Containing Compartments
- H. Inadome, Y. Noda, H. Adachi, K. Yoda
- Biology, Computer ScienceMolecular and Cellular Biology
- 1 September 2005
The data suggest that Svp26 has a novel role in retention of a subset of membrane proteins in the early Golgi compartments, which are physically separated in the Saccharomyces cerevisiae cell.
Localization of GDP-mannose transporter in the Golgi requires retrieval to the endoplasmic reticulum depending on its cytoplasmic tail and coatomer
Results indicate that GMT escapes from delivery to the vacuole by recycling to the endoplasmic reticulum and retrieval requires the lysine-rich C-terminal tail that can bind to the COPI coat.
Binding of Sly1 to Sed5 enhances formation of the yeast early Golgi SNARE complex
In vitro formation of this complex on different membranes in yeast lysate was enhanced by the addition of recombinant Sly1, indicating that binding of Sly1 to Sed5 enhances trans-SNARE complex formation.