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Acetone, Isopropanol, and Butanol Production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum.
Thirty-four strains representing 15 species of anaerobic bacteria were screened for acetone, isopropanol, and n-butanol (solvent) production. Under our culture conditions, several strains ofExpand
Improved media for growth and aerotolerance of Campylobacter fetus.
The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement ofExpand
Sequence determination of human papillomavirus type 6a and assembly of virus-like particles in Saccharomyces cerevisiae.
Human papillomavirus 6a (HPV6a), the most abundant HPV6 subtype, was detected in a vulvar condyloma acuminatum. The complete genome of HPV6a was cloned, and its DNA sequence was shown to be over 97%Expand
Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae.
Recombinant major capsid protein, L1 (M(r) = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression systemExpand
Acidic Conditions Are Not Obligatory for Onset of Butanol Formation by Clostridium beijerinckii (Synonym, C. butylicum).
Factors that may initiate the metabolic transition for butanol production were investigated in batch cultures of Clostridium beijerinckii (synonym, Clostridium butylicum) VPI 13436. CulturesExpand
Human papillomavirus type 11 (HPV-11) neutralizing antibodies in the serum and genital mucosal secretions of African green monkeys immunized with HPV-11 virus-like particles expressed in yeast.
It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimentalExpand
Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter.
  • J. Li, S. Wang, +6 authors G. Rao
  • Biology, Medicine
  • Biotechnology and bioengineering
  • 20 October 2000
Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector andExpand
Continuous culture study of the expression of hepatitis B surface antigen and its self‐assembly into virus‐like particles in Saccharomyces cerevisiae
We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24s monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose‐limitedExpand
Adenine Quantitation in Yeast Extracts and Fermentation Media and Its Relationship to Protein Expression and Cell Growth in Adenine Auxotrophs of Saccharomyces cerevisiae
We have described a method to reliably measure the free adenine content of yeast extract powders or the adenine concentrations found in chemically‐defined and complex fermentation samples. ThisExpand
Studies of the microaerophilic nature of Campylobacter fetus subsp. jejuni. II. Role of exogenous superoxide anions and hydrogen peroxide.
The addition of bovine superoxide dismutase to Brucella broth or Brucellar agar greatly echanced the oxygen tolerance of Campylobacter fetus subsp. jejuni strain H840 (ATCC 29428). Catalase alsoExpand