H. R. Buckley

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A chemically defined medium composed of 6 amino acids, biotin, inorganic salts and glucose for the growth of yeast and mycelial phases of Candida albicans at 25 degrees C and 37 degrees of C respectively was developed based on the aminopeptidase(s) profile of the fungus. This medium has proved successful in maintaining the growth characteristics of both(More)
Candida albicans exhibits hemolytic activity when grown on glucose-enriched blood agar. This activity is present on intact organisms, and it is secreted into the culture medium. Hemoglobin released from lysed erythrocytes can restore the transferrin-inhibited growth of C. albicans. We conclude that C. albicans expresses a hemolytic factor which allows it to(More)
BACKGROUND Invasive candidiasis is a major nosocomial infection that is difficult to diagnose. Few biochemically defined markers of invasive candidiasis are known. Initial findings suggested that the presence of candida enolase in the blood may be a novel marker for invasive candidiasis. METHODS We tested 170 patients at high risk for invasive candidiasis(More)
Three monoclonal antibodies, designated A2C7, C2C7, and F19, were produced which recognize proteins from Candida albicans. All are of the immunoglobulin G1 heavy chain and kappa light chain class. A2C7 and C2C7 immunoprecipitated three proteins contained in a partially purified fraction (region A) of a mycelial cytoplasmic extract of C. albicans. The(More)
We evaluated the RapID Yeast Plus System using 117 fresh and frozen clinical yeast isolates. The Uni-Yeast-Tek System was used to establish the correct identification. The Vitek System was used as the arbiter for any discrepant results, along with morphology. Of 117 isolates tested, the RapID Yeast Plus System identified 96.6% correctly. The RapID Yeast(More)
The alkyl glycerol ether rac-1-O-dodecylglycerol inhibited the growth of members of two genera of yeasts, Candida and Cryptococcus, and was strongly synergistic with amphotericin B. At one-half its MIC, dodecylglycerol decreased the MIC of amphotericin B by as much as 80-fold. This high degree of synergism between dodecylglycerol and amphotericin B was(More)