H-P Schuster

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We constructed and tested a series of cloning vectors designed to facilitate protein production and purification in Dictyostelium discoideum (Dd). These vectors carry the origin of replication of the Dd high-copy-number plasmid Ddp2, expression cassettes consisting of the strong, constitutive actin (act15) or the inducible discoidin (disI gamma) promoters,(More)
OBJECTIVES To evaluate the safety and potential efficacy of antithrombin III (AT III) in reducing mortality in patients with severe sepsis. DESIGN Prospective, randomized, placebo-controlled, double-blind, phase II, multicenter, multinational clinical trial. SETTING Seven academic medical center intensive care units (ICU) in Belgium, Denmark, the(More)
The interaction of the nucleotide-free molecular chaperone DnaK (Hsp70) from Escherichia coli with nucleotides was studied under equilibrium and transient kinetic conditions. These studies used the intrinsic fluorescence signal of the single tryptophan residue (Trp102) of DnaK, or of novel fluorescent nucleotide analogs of ADP and ATP,(More)
In spite of good correlations between cardiac output measurements by impedance and established invasive procedures (dye- and thermo-dilution) reported by numerous authors it is doubtful uptil now whether calculations of stroke volume according to the formula of Kubicek et al. (1974) can provide absolutely reliable results. The origin of the dz/dt curve and(More)
Sepsis and its associated complications of disseminated intravascular coagulation (DIC) and multiple organ dysfunction syndrome (MODS) continue to be a major cause of morbidity and mortality. Improved detection of all forms of DIC is essential to assure earlier diagnosis. Studies already indicate that the therapeutic use of antithrombin (AT) concentrate may(More)
Objectives: To evaluate the safety and potential efficacy of antithrombin III (AT III) in reducing mortality in patients with severe sepsis. Design: Prospective, randomized, placebo-controlled, double-blind, phase II, multicenter, multinational clinical trial. Setting: Seven academic medical center intensive care units (ICU) in Belgium, Denmark, the(More)
Basic therapy of acute renal failure (a.r.f.) consists of normalization of body fluids, substitution of albumin losses, high dose furosemide and parenteral nutrition. Extracorporeal hemodialysis is indicated in postoperative a.r.f. with oligo-anuria. Hemodialysis should be performed in I.C.U. The prognosis of a.r.f. is still poor. Prophylaxis is most(More)
In 68 patients, subxiphoidal, percordial, and suprasternal echocardiography was used to trace the Swan-Ganz catheter during its passage to the pulmonary artery and to localize its position. The localization of the catheter could be exactly identified in 62 out of 68 patients in whom we managed to obtain a suprasternal echocardiogram. In one patient, the(More)
Objective: To evaluate the applicability of the Simplified Acute Physiology Score (SAPS II) for coronary care patients. Design: Prospective observational cohort study. Setting: Medical ICU of a community teaching hospital. Patients: 1587 consecutive patients admitted over a period of 18 months. Measurements and main results: Patients were divided in two(More)