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A simple and rapid radioimmunoassay (RIA) without extraction or purification was developed for Total Estrogen (conjugated and unconjugated estrogen) in urine from normal men and nonpregnant women. Antiserum used to RIA was produced by immunizing rabbits with estriol-16 alpha-glucuronide (E3-16-G)-BSA conjugates. Antiserum to E3-16-G.BSA significantly(More)
A simple and practical radioimmunoassy (RIA) for pregnanediol without any pretreatment of urine specimens such as hydrolysis and/or extraction was developed. Antisera were produced by immunizing rabbits with pregnanediol-3-glucuronide (P-diol-3-G)-BSA conjugates and P-diol-3-hemisuccinate-BSA conjugates. The specificities of the antisera were examined by(More)
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