H. Kalervo. Väänänen

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BACKGROUND AND AIMS Serum levels of gastrin-17 (S-G-17) and pepsinogen I (S-PGI) are biomarkers of gastric antral and corpus mucosa, respectively. In a prospective multicentre investigation, we determined whether these tests, together with the assay of Helicobacter pylori antibodies, are a non-endoscopic tool for the diagnosis of atrophic gastritis. (More)
p38 belongs to a family of mitogen-activated protein kinases, which transfer extracellular signals into intracellular responses. p38 is also frequently detected in clinical breast cancer specimens, but its role as a prognostic factor is not known. Of the various p38 isoforms, p38β has been shown to mediate the in vitro invasiveness of breast cancer cells(More)
Incubation of human colonic contents with various ethanol concentrations (2.75-44 mM) in vitro at 37 degrees C resulted in significant accumulation of acetaldehyde--a toxic and highly reactive compound. At pH 9.6, all samples produced notable acetaldehyde concentrations (58 (13) microM; mean (SEM)) even from the lowest (2.75 mM) ethanol concentration, and(More)
Using quantitative fluorescence microscopy of red cells loaded non-disruptively with 1-2.5 mmol/l cells of fura-2, we examined the distribution of the incorporated free chelator among and within individual cells. Cytoplasmic hemoglobin quenched the effective fluorescence yield of fura-2 by a factor of about 100. All red cells were found to fluoresce upon(More)
This study was undertaken to evaluate the success of triple therapy in peptic ulcer patients and ulcer relapses. One hundred and one consecutive Helicobacter pylori-positive peptic ulcer patients were assigned to an open trial with 2 weeks of treatment with colloidal bismuth subcitrate, amoxicillin, and metronidazole. At the 6-week follow-up only 1 duodenal(More)
The bone resorbing cells, osteoclasts, express high levels of carbonic anhydrase II (CA II) and vacuolar H(+)-ATPase (V-ATPase) during bone resorption. We have used antisense RNA and DNA molecules targeted against CA II, and against 16- and 60-kD subunits of vacuolar H(+)-ATPase (V-ATPase), to block the expression of these proteins in vitro. Osteoclastic(More)
BACKGROUND Teriparatide is a potent anabolic agent for severe osteoporosis. OBJECTIVES A primary objective of this retrospective study was to define the efficacy of teriparatide in terms of bone mineral density (BMD) changes and relief of back pain in clinical practice. METHODS The patient population comprises 119 osteoporotic patients treated with(More)
 Osteopetrosis in laboratory animals is a metabolic bone disease characterized by increased skeletal mass. It is inherited as an autosomal recessive and results from a defect in the development and/or function of osteoclasts. We studied two enzymes essential for bone resorption, carbonic anhydrase II isoenzyme (CA II) and H+-ATPase, in osteoclasts from four(More)
The effect of phenobarbital pretreatment on acinar distribution of microsomal drug metabolizing enzymes was investigated by analysis of periportal (pp) and perivenous (pv) enriched rat hepatocytes isolated by collagenase gradient perfusion. In untreated animals the activities of cytochrome P-450, NADPH-cytochrome c reductase and microsomal ethanol oxidation(More)
The effect of ethanol on hepatic regeneration after partial hepatectomy was studied. Ethanol was administered in a nutritionally adequate liquid diet 4 times daily by gastric intubation. The dose of ethanol was selected to maintain a continuous level of ethanol in the animals throughout the experiment. Treatment was started 24 hours before the operation and(More)