Learn More
TaqMan™ real time PCR assays were designed for each of the non-O157 STEC O serogroups most commonly associated with human illness: O26, O45, O91, O103, O111, O113, O121, O128, and O145. The nine RT-PCR assays can be run as single assays when a known pathogen is of concern, or multiplexed in three reactions, to quickly screen for the most clinically relevant(More)
The objective of this study was to develop a rapid, simple method for enhanced detection and isolation of low levels of Escherichia coli O157:H7 from leafy produce and surface water using recirculating immunomagnetic separation (RIMS) coupled with real-time PCR and a standard culture method. The optimal enrichment conditions for the method also were(More)
The paper proposes a novel pilot-symbol-aided (PSA) technique for fading compensation of digital signals in the mobile environments. In a PSA system, the data sequence at the transmitter is divided into frames of data. A pilot symbol from a known pseudoradom-symbol sequence is inserted periodically into a frame of data symbol for transmission. In a(More)
Two automated platforms using immunomagnetic separation technology were compared for detecting and recovering Escherichia coli O157 in ground beef and sprouts and Shigella flexneri in green onions. The foods were inoculated with <20 CFU/25 g and tested at 5 and 24 h postincubation. Immunomagnetic beads were mixed with food enrichments, processed through the(More)
The four components of hemoglobin from the rainbow trout (Salmo gairdneri) have been isolated. The oxygen affinities of the first two components eluted from the DEAE-cellulose column have much smaller pH dependencies than the last two components. These components have very low O2 affinities at low pH. The effect of pH on the equilibrium and kinetics of(More)
– The paper studies the bit-error-rate (BER) performance of a pilot-symbol-aided (PSA) 16QAM system with an N-branch postdetection selection combining diversity reception over multipath fading channels. Both pilot symbols and data symbols are used to compensate for the fading distortion. Computer simulation results have shown that the use of diversity(More)