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The structure of human rhinovirus-14 3C protease (3Cpro) has been determined at 2.3 A resolution and refined to an R factor of 0.22. This cysteine protease folds into two topologically equivalent six-stranded beta barrels and in this sense is similar to trypsin-like serine proteases. However, there are differences in the lengths and positioning of(More)
Site-directed mutagenesis has been used to explore the role of two carboxylates in the active site of histidine decarboxylase from Lactobacillus 30a. The most striking observation is that conversion of Glu197 to either Gln or Asp causes a major decrease in catalytic rate while enhancing substrate binding. This is consistent with models based on X-ray(More)
We have constructed a family of novel in vitro transcription vectors in which functional T3, T7 and SP6 RNA polymerase promoters are arranged in tandem and directed towards a multiple cloning site. This prototype vector, named pTRIPLEscript, permits the transcription of one strand of a DNA insert by any of the three commonly used bacteriophage RNA(More)
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