H. Cruzblanca

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This study is an investigation into the modulatory effects of heparin, a component of the extracellular matrix that binds to dihydropyridine receptors, on contraction and Ca2+ channels in frog skeletal muscle. Using tension and Ca2+ signal measurements in single intact skeletal muscle cells we have found that heparin (100–200 μg ml-1) substantially(More)
The effect of temperature on electrical interactions between antidromically stimulated motoneurons and dorsal root afferents was studied in the isolated and hemisected spinal cord of the frog, superfused with Ringer in which Ca2+ was equimolarly replaced by Co2+ or Mn2+ to suppress chemical synaptic transmission. Suction electrodes were used for stimulating(More)
1. Transmembrane ion movements upon sodium-pump inhibition were studied in identified neurons of the subesophageal ganglia of Helix aspersa. A two-microelectrode, voltage-clamp technique was used to measure transmembrane currents. Changes in intracellular Na+, K+, and Ca2+ concentrations were measured, in unclamped neurons, with Na(+)-sensitive(More)
This study aimed to assess the effects of the antidepressant drug imipramine (IMI) on the neuronal M-type K+ current (IK(M)). We show that IMI reversibly reduces IK(M) with an IC50 of 7 microM. The V0.5 and slope factor of the steady state activation curve remained unchanged after IMI, indicating a mode of action that is voltage insensitive for blocking the(More)
The effects of trifluoperazine hydrochloride (TFP), a calmodulin antagonist, on L-type Ca2+ currents (L-type ICa2+) and their Ca2+-dependent inactivation, were studied in identifiedHelix aspersa neurons, using two microelectrode voltage clamp. Changes in [Ca2+]i were measured in unclamped fura-2 loaded neurons. Bath applied TFP produced a reversible and(More)
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