H. Brian Halsall

Learn More
Electrochemical biosensors combine the sensitivity of electroanalytical methods with the inherent bioselectivity of the biological component. The biological component in the sensor recognizes its analyte resulting in a catalytic or binding event that ultimately produces an electrical signal monitored by a transducer that is proportional to analyte(More)
This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low-volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Microfluidic components have been developed and integrated to construct a microfluidic biochemical detection(More)
Immunosensing microfluidic devices are reviewed. Devices are commonly fabricated in glass, silicon, and polymers, with polymers seeing greater attention in recent years. Methods have been developed to immobilize antibodies and other molecules and resist non-specific adsorption through surface modification. The most common detection method is fluorescence,(More)
The concept of a simultaneous dual analyte immunoassay based on two different metal ion labels is demonstrated. The model system consists of two proteins, human serum albumin (HSA) and immunoglobulin G (IgG). Bismuth and indium ions have been coupled to these proteins through the bifunctional chelating agent diethylenetriamine-pentaacetic acid (DTPA). A(More)
Sodium dodecyl sulphate (SDS) was used to weaken both the electrostatic and the hydrophobic interactions during collagen fibrillogenesis in vitro. The rate and extent of fibril formation as well as fibril morphology were affected by SDS concentration. Both the formation of large fibrils at 0.3 mM-SDS and the complete cessation of fibril formation at 0.5(More)
Crystals of alpha 1-acid glycoprotein have been grown reproducibly from delipidated protein in the presence of chlorpromazine. The crystals are large hexagonal prisms of space group either P622 or P6(2)22 and the unit cell dimensions are a = b = 101 A and C = 201 A. The unit cell is very highly hydrated and is nearly 80% solvent. It contains one molecule of(More)
Orosomucoid (OMD) contains complex bi-, tri- and tetra-antennary glycan chains. Subfractionation of OMD into three molecular variants using concanavalin A lectin chromatography is based on variations in these complex structures. Standard h.p.l.c. profiles have been developed to analyse the percentage and distribution of the glycoforms present at each(More)
Alpha(1)-acid glycoprotein (AGP) consists of 183 amino acid residues and 5 carbohydrate chains and binds to basic and neutral drugs as well as steroid hormones. We investigated the structural properties and ligand-binding capacity of AGP under mild acidic conditions and its interactions with liposomes prepared from neutral or anionic lipids and the neutral(More)
A simple, fast and sensitive method was developed to verify the presence of the sialyl Lewis(x) antigen on an N-linked glycoprotein. High performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was used to identify which of the five N-linked glycosylation sites of human plasma alpha1-acid-glycoprotein (orosomucoid, OMD) contain the(More)