H. Boettcher

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After reconsidering the physicochemical mechanisms involved in the so-called degeneration methods for the demonstration of axons and nerve terminals, the method of Eager was fundamentally modified in order to stabilize the staining process. This resulted in a simple and reliable method which stains degenerating terminals and lysosomes with a high degree of(More)
The development of glutamate decarboxylase (GAD)-like immunoreactivity was studied in albino rats. GAD-positive structures appeared and accumulated in a characteristic temporo-spatial sequence: from embryonic day 18 onwards in lamina (L) I and subplate; after birth in L I-II, L IV-V and deep L VI including white matter; after two weeks in superficial L I, L(More)
A method has been elaborated by which degenerating axons can be selectively impregnated with silver. Based on reconsideration of the physicochemical mechanisms of the degeneration methods it takes advantage of physical developers over the chemical ones. The staining procedure is applied to frozen sections of brains fixed with formol. It consists of 6 steps:(More)
A novel method facilitates the determination of O2 content in the gas phase and in the culture medium of closed cell culture systems: Total O2 was swept out for 3 min by a stream of N2 under atmospheric pressure and carried to the measuring unit of a PBI Dansensor TIA-111-LV oxygen sensor, creating a peak-shaped signal, which was integrated over time. From(More)
For total-body irradiation (TBI) using the translation method, dose distribution cannot be computed with computer-assisted three-dimensional planning systems. Therefore, dose distribution has to be primarily estimated based on CT scans (beam-zone method) which is followed by in vivo measurements to ascertain a homogeneous dose delivery. The aim of this(More)
PURPOSE To evaluate the influence of tumor characteristics and irradiation on blood selenium concentrations and their course during radiotherapy. MATERIAL AND METHODS Selenium Levels were determined at various times during radiotherapy (I: beginning, II: mid, III: end, IV: 6 weeks after) by atomic absorption spectrometry in whole blood. The values were(More)
In der Literatur finden sich wenig Informationen über den Einfluß der adjuvanten postoperativen Radiotherapie auf die psychische Belastung brusterhaltend operierter Brustkrebspatientinnen. In der täglichen Praxis kann jedoch Sorge und Angst im Zusammenhang mit der Bestrahlung beobachtet werden. Ziel unserer Untersuchung war es, den allgemeinen und den(More)
In patients with severe hypothermia and cardiac arrest, active rewarming is recommended by extracorporeal circulation with cardiopulmonary bypass. The current guidelines for resuscitation of the European Resuscitation Council now include the recommendation regarding patients with hypothermia remaining comatose after initial resuscitation to accomplish an(More)
A new, inexpensive technique facilitates the observation of long-lasting effects (> 2 h) of thermogenic agents on cell cultures. A special microcatheter enables addition of hormones or pharmacologic agents to biological samples during microcalorimetric analysis using a ThermoMetric ThermalActivityMonitor without removing the sample ampoules from the(More)
Für Patienten mit schwerer Hypothermie und Kreislaufstillstand wird eine aktive Wiedererwärmung mithilfe der extrakorporalen Zirkulation an der Herz-Lungen-Maschine empfohlen. Neu ist in den aktuellen Leitlinien des European Resuscitation Council zur kardiopulmonalen Reanimation die Empfehlung, bei komatösen Patienten mit vorbestehender Hypothermie, die(More)