H. B. Scholtens

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This study describes the "plasma" disappearance and biliary excretion of injected purified canine intestinal alkaline phosphatase in the isolated perfused rat liver and in the intact rat. The plasma disappearance curve of the enzyme showed, apart from an initial rapid phase, a secondary phase, which may result from "exocytosis" of interiorized canine(More)
Glycoproteins represent a wide variety of macromolecules with important physiological functions. Characteristic variations in carbohydrate composition and plasma concentration of these proteins may occur during pathological conditions. Steady-state plasma concentrations are determined by release from normal or diseased tissues and simultaneous clearance(More)
After intravenous administration to rats, the distribution of injected canine intestinal alkaline phosphatase in the liver was studied morphologically by means of histochemical and cytochemical methods on liver sections and pellets of homogenized livers. The interference of various sugars, sugar polymers and glycoproteins with the receptor-bound injected(More)
The beta-endorphin (beta E) fragment des-Tyr1-gamma-endorphin (DT gamma E, beta E-(2-17)) has been reported to interact with neuroleptic binding in vivo but not in vitro. We have attempted to replicate the in vivo experiments and extended the work to include conditions in which des-enkephalin-gamma-endorphin (DE gamma E, beta E-(6-17)) exhibited behavioral(More)
H-Pro-Leu-Gly-NH2 (PLG) was labeled with 3H-leucine by catalytic tritiation of H-Pro-methylallylgylcyl-Gly-NH2 and purified by ion-exchange chromatography. Metabolism of 3H-PLG in rat and human plasma was investigated by reversed phase-paired ion high-pressure liquid chromatography. All possible metabolites could be completely separated within 25 min.(More)
1. Dog intestinal alkaline phosphatase (IAP), an asialoglycoprotein, appeared to be a good marker for the histochemical detection of the galactose specific binding protein in cryostat sections of rat liver. 2. Binding of IAP to the receptor is optimal at neutral or slightly alkaline pH values. The binding could be inhibited by galactose and galactose(More)
The plasma disappearance, metabolism and uptake in the brain of [3H-Phe4]-DT gamma E and [3H-Lys9]-DE gamma E were investigated following systemic administration of these neuroleptic-like peptides to rats. 3H-DT gamma E, 3H-DE gamma E and their radioactive metabolites in plasma and brain extracts were determined by reversed-phase HPLC. Plasma disappearance(More)
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