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After reconsidering the physicochemical mechanisms involved in the so-called degeneration methods for the demonstration of axons and nerve terminals, the method of Eager was fundamentally modified in order to stabilize the staining process. This resulted in a simple and reliable method which stains degenerating terminals and lysosomes with a high degree of(More)
BACKGROUND The success of percutaneous intervention in peripheral arterial disease is limited by restenosis. The aim of the present pilot study was to evaluate a novel method of local drug delivery. METHODS AND RESULTS This randomized multicenter study with blinded reading enrolled 87 patients in Rutherford class 1 to 4 with occlusion or hemodynamically(More)
The development of glutamate decarboxylase (GAD)-like immunoreactivity was studied in albino rats. GAD-positive structures appeared and accumulated in a characteristic temporo-spatial sequence: from embryonic day 18 onwards in lamina (L) I and subplate; after birth in L I-II, L IV-V and deep L VI including white matter; after two weeks in superficial L I, L(More)
A method has been elaborated by which degenerating axons can be selectively impregnated with silver. Based on reconsideration of the physicochemical mechanisms of the degeneration methods it takes advantage of physical developers over the chemical ones. The staining procedure is applied to frozen sections of brains fixed with formol. It consists of 6 steps:(More)
A novel method facilitates the determination of O2 content in the gas phase and in the culture medium of closed cell culture systems: Total O2 was swept out for 3 min by a stream of N2 under atmospheric pressure and carried to the measuring unit of a PBI Dansensor TIA-111-LV oxygen sensor, creating a peak-shaped signal, which was integrated over time. From(More)
PURPOSE To evaluate magnetic resonance (MR) imaging-controlled laser-induced thermotherapy (LITT) in the treatment of recurrent head and neck tumors. MATERIALS AND METHODS Six patients with recurrent nasopharyngeal tumors (squamous cell carcinoma [n = 4], pleomorphic adenoma [n = 2]) underwent LITT with local anesthesia. A 7-F introducing sheath was(More)
Histochemical localization of two lectins, Ulex europaeus agglutinin-I (UEA-I) and Tetragonolobus purpureus (TPA), was studied in the olfactory bulb of adult rats. In contrast to TPA, UEA-I detected a fucosylated glycoprotein that is only present in the surface membranes of olfactory sensory cells including the whole course of their neurites up to the final(More)
Soluble HLA (sHLA) antigens were measured in a panel of 50 renal and 50 cardiac graft recipients by a quantitative ELISA over periods ranging between 2 months and 3 years. 72% of the renal patients and 68% of the cardiac graft patients experienced episodes of acute rejection during the observation period. sHLA were elevated to over 2-5 times the normal(More)