Gustavo M. A. Lima

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Following burgeoning genomic and transcriptomic sequencing data, biochemical and molecular biology groups worldwide are implementing high-throughput cloning and mutagenesis facilities in order to obtain a large number of soluble proteins for structural and functional characterization. Since manual primer design can be a time-consuming and error-generating(More)
The current Zika virus (ZIKV) outbreak became a global health threat of complex epidemiology and devastating neurological impacts, therefore requiring urgent efforts towards the development of novel efficacious and safe antiviral drugs. Due to its central role in RNA viral replication, the non-structural protein 5 (NS5) RNA-dependent RNA-polymerase (RdRp)(More)
Gastric volvulus is a rare entity that was first described by Berti in 1866 while performing a necropsy. In 1896, Berg first established the diagnosis during the operation. In 1904, Borchardt described the classic triad of epigastric distension with pain, difficulty for the nasogastric tube to pass and nausea without vomiting. A case of a female patient(More)
A case of iatrogenic left ventricular pseudoaneurysm in a male patient, with a diagnosis of pericarditis, is presented. The patient developed severe complications after an attempted diagnostic and therapeutic pericardiocentesis. Six months later, after corrective surgery, the pseudoaneurysm recurred. The patient had a second operation and two connections to(More)
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