Gustavo Cerda

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The combination of accessible embryology and forward genetic techniques has made zebrafish a powerful model system for the study of vertebrate development. One limitation of genetic analysis is that the study of gene function is usually limited to the first developmental event affected by a gene. In vivo electroporation has recently matured as a method for(More)
Within the vertebrate lineage, a high proportion of duplicate genes have been retained after whole genome duplication (WGD) events. It has been proposed that many of these duplicate genes became indispensable because the ancestral gene function was divided between them. In addition, novel functions may have evolved, owing to changes in cis-regulatory(More)
Different studies have demonstrated the importance of micronutrients, such as vitamins, for normal adult brain function and development. Vitamin C is not synthesized in the brain, but high levels are detected in this organ because of the existence of specific uptake mechanisms, which concentrate ascorbic acid from the bloodstream to the cerebrospinal fluid(More)
Sonic hedgehog (Shh)/Gli pathway plays an important regulatory role on the neuroepithelial cells (NEc) proliferation in the dorsal regions of the developing vertebrate Central Nervous System. The aim of this paper was to analyze the effect of the Shh/Gli signaling pathway activation on the proliferation dynamics and/or the spatial organization of the NEc(More)
In this paper we present an efficient way to both compute and extract salient information from trace transform signatures to perform object identification tasks. We also present a feature selection analysis of the classical trace-transform functionals, which reveals that most of them retrieve redundant information causing misleading similarity measurements.(More)
In this report, we describe a successful protocol for isolating and expression-profiling live fluorescent-protein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in(More)
During development of the central nervous system, neurons pass through windows of vulnerability to environmental factors. Two windows are gestational day (G) 7 (during gastrulation) and G12 (early neuronal generation). The present study examined the effects of ethanol exposure at these times on social behavior and on the number of neurons in the central(More)
Colobanthus quitensis is the only native dicotyledoneae in the Antarctic; therefore, it is the object of ample research regarding tolerance mechanisms to its extreme habitat. This species also represents a wide geographical distribution, from 68°S to 17°N and from 0 m a.s.l. in the south to 4200 m a.s.l. in the north of its distribution area. As the(More)
organization is its bilateral symmetry, most evident at the level of the vertebrae and skeletal muscles. Here, we show that Rere (Atrophin2)-deficient mouse embryos form asymmetrical somites in a temporally defined window. During the time period spanning the formation of somites 7 to 13 in Rere mutants, there is a lack of left–right coordination of the(More)
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