Gurmit S. Aulakh

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In a prelminary communication, we described the establishment of a continuous human myeloid cell line (HL-60). Here we report the detailed properties of this cell line and document its derivation from the peripheral blood leukocytes of a patient with acute promyelocytic leukemia. As characterized by light and electron microscopy, the predominant cell type(More)
Similarities in the biochemical and serological characteristics of sterol-nonrequiring acholeplasmas (mycoplasmas) have created problems in the taxonomy and classification. The present study was designed to compare the genetic characteristics of two unclassified acholeplasma strains with those of the established speciesAcholeplasma. Radiolabeled DNA probes(More)
A [3H[cDNA probe synthesized from the RNA genome of Rauscher murine leukemia virus (MuLVR) and purified by hybridization to MuLVR70S RNA was hybridized to DNA from human normal and hemotopoietic neoplasia tissues. This cDNA hybridized completely to its homologous 70S RNA and was free of self-complementary sequences. Sequences complementary to MuLVR cDNA(More)
Lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral B lymphocytes (B cells) harboring endogenous Epstein-Barr virus or by superinfecting peripheral lymphocytes with exogeneous Epstein-Barr virus. Results of extensive studies aimed at identifying type C(More)
L-forms from a Corynebacterium were induced in hyperosmolar media and gradually adapted to normal osmolarity over a period of two years. During this adaptation several morphologic L-form variants derived from the L-form cultures and were serologically identified as Acholeplasma laidlawii A. The possibility that the bacterial and L-form cultures were(More)
Latent murine cytomegalovirus (MCMV) infection of BALB/c mouse spleens was studied using several methods including an explant tissue culture technique, co-cultivation on allogeneic and syngeneic cell cultures and nucleic acid hybridization. BALB/c mice experience latent infection which persists for at least 6 months and involves only a small fraction of(More)
This study compares the intraspecies genetic relatedness among strains of two established species of Acholeplasma. Radiolabelled DNA probes were prepared from three strains of Acholeplasma laidlawii and two strains of Acholeplasma axanthum, by using the nick translation method. The labelled DNA probes of these two strains were hybridized to an excess of(More)
3H-labeled Acholeplasma DNA probes were generated in vitro by the nick-translation method and used to determine the nucleotide sequence homology among the type strains of the eight currently recognized species of Acholeplasma. Very little nucleotide sequence homology (less than or equal to 18%) was found among the eight species, with heteroduplexes showing(More)
Radiolabeled DNA probes prepared in vitro by the nick translation method were used to determine the nucleotide sequence homology among the eight established and one unclassified species of Acholeplasma. Very little DNA homology (2 to 21 percent) was found among these nine distinct species and the heteroduplexes showed at least 15 percent mismatching as(More)