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A SMART cDNA library from spleen of large yellow croaker (Pseudosciaena crocea) stimulated by poly I:C was constructed. A total of 1039 clones from the library were single-pass sequenced and compared with known sequences in the GenBank database. Of those expressed sequence tags (ESTs), 607 were identified as orthologs of known genes in the GenBank databases(More)
Antigenic peptides presented on MHC class I molecules to cytotoxic T-cells are generated in the cytosol by the 20S proteasome. Two activators PA28-alpha and PA28-beta, which are inducible by interferon-gamma (IFN-gamma), activate the latent 20S proteasome, thus playing an important role in the processing of MHC class I antigen. Molecular properties and(More)
A multiresidue method was developed based on extraction of 10 g sample with 10 mL acetonitrile and subsequent liquid-liquid partitioning formed by adding 4 g MgSO₄ plus 1 mL chloroform. During the partitioning process, the extraction recoveries of polar analytes were found to be essentially determined by the acetonitrile content in the aqueous phase. The(More)
CD59 is a widely distributed membrane-bound inhibitor of the cytolytic membrane attack complex (MAC) of complement. Here, the cDNA of a CD59 analogue was cloned from large yellow croaker (Pseudosciana crocea), a marine fish (LycCD59), by expressed sequence tags (EST) and RACE techniques. The open reading frame (ORF) of 351 nucleotides (nt) of LycCD59(More)
Scutellaria baicalensis Georgi is a well-known medicinal plant widely used in China and other East Asian countries. High performance liquid chromatography combined with diode array detection and electrospray ion trap mass spectrometry was used to determine the flavonoid profile of S. baicalensis. Under the optimized experiment conditions, 32 flavonoids were(More)
In this text we present a series of LC-MS/UV-based strategies for on-line structure identification of unknown flavone glycosides in Dracocephalum tanguticum. The aglycone portion, the glycosylation position, the order of sugars and the identity of sugars of each glycoside were unambiguously identified. The new strategy--identifying the partial structure of(More)
Scutellaria baicalensis Georgi (S. baicalensis), a perennial herb of the Labiatae family, is a well-known traditional Chinese medicine. In the present study, a comprehensive qualitative analysis of flavonoids in the stems and leaves of S. baicalensis was performed. Under the optimized experimental conditions, 21 flavonoids were clearly detected. 17 of them(More)
Novel sample preparation approaches for HPLC bioanalysis based on the phenomenon that acetonitrile can be separated from water by adding salts or cooling at subzero temperatures have been reported. These two methods are superior to conventional liquid-liquid extraction since the separated acetonitrile phase can be directly injected to the RP-LC system.(More)
A simple and accurate method has been developed to simultaneously separate and determine 10 bioactive compounds in citrus fruits by high-performance liquid chromatography coupled with diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS). This HPLC assay was performed on a reversed-phase C18 column with acetonitrile and 0.1%(More)
High selectively catalytic conversion of lignin-based phenols (m-cresol, p-cresol, and guaiacol) into para-/m-xylene was performed over Pt/HZSM-5 through hydrodeoxygenation and in situ methylation with methanol. It is found that the p-/m-xylene selectivity is uniformly higher than 21%, and even increase up to 33.5% for m-cresol (with phenols/methanol molar(More)