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Two new flavonoids, pauciaurone A (1) and pauciisoflavone A (2), together with three known isoflavones (3–5), were isolated from the stems of Garcinia paucinervis. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compounds 1 and 2 were tested for their cytotoxicities against five human tumor cell lines(More)
The vacuolar ATPase (V-ATPase) is a multifunctional enzyme that consists of several subunits. Subunit B is a part of the catalytic domain of the enzyme. The result of the RT-PCR suggested that the V-ATPase B subunit is a ubiquitous gene. 24 h after the larvae were infected with the Bombyx mori nucleopolyhedrovirus (BmNPV), the expression level of the(More)
Gene nanos is a maternal posterior group gene required for normal development of abdominal segments and the germ line in Drosophila. Expression of nanos-related genes is associated with the germ line in a broad variety of other taxa. In this study, the 5'-RACE method and the in silico cloning method are used to isolate the new nanos-like gene of Bombyx mori(More)
Investigation of an organic extract of the stems of Garcinia bracteata led to the isolation of two new flavones, named bracflavones A (1) and B (2). Their structures were elucidated by spectroscopic evidence. The compounds feature an isopropoxy group at C-3 compared with related known flavonoids isolated from the genus Garcinia. Compounds 1 and 2 were(More)
The nanos gene encodes a zinc-finger protein which is required for the migration and differentiation of primordial germ cells as well as for their fate maintenance. In this study, a 1913 bp nanos gene was cloned and characterized in silkworm (Bombyx mori). RT-PCR and Western blot analysis showed that the nanos was expressed in developing embryos and various(More)
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