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In Vitro Bacterial Polysaccharide Biosynthesis: Defining the Functions of Wzy and Wzz
TLDR
A chemical approach was presented which enabled reconstitution of the E. coli O-polysaccharide biosynthesis pathway in vitro, providing a well-defined system for further underpinning molecular details of this biosynthetic pathway.
Enzymatic route to preparative-scale synthesis of UDP–GlcNAc/GalNAc, their analogues and GDP–fucose
TLDR
GDP–fucose is constructed by one bifunctional enzyme l- fucose pyrophosphorylase (FKP) via two reactions and other UDP–GlcNAc/GalNAc analogues can also be prepared depending on the tolerance of these enzymes to the modified sugar substrates.
Warmer temperature accelerates methane emissions from the Zoige wetland on the Tibetan Plateau without changing methanogenic community composition
TLDR
Temperature increases methane emissions in alpine wetlands, while vegetation contributes significantly to methanogenic community composition and is associated with methane emissions, which furthers a global warming feedback loop.
EFFECT OF FARNESOL ON PENICILLIUM DECUMBENS’S MORPHOLOGY AND CELLULASE PRODUCTION
TLDR
It is shown that scanning electron microscopy revealed that farnesol promoted the growth of hyphae, making possible and facilitating a higher yield of cellulase secretion, and enhanced interaction with the substrate in fermentation led to greater cellulase production.
Synthesis of Rare Sugars with L-Fuculose-1-phosphate Aldolase (FucA) from Thermus thermophilus HB8.
A one-pot, four-enzyme approach yields the rare sugars (D)-psicose (III), (D)-sorbose (IV), (L)-tagatose, and (L)-fructose in the presence of the thermophilic source-derived title aldolase as the
Defining Function of Lipopolysaccharide O-antigen Ligase WaaL Using Chemoenzymatically Synthesized Substrates*
TLDR
The present work represents an in vitro systematic investigation of the WaaL function using a chemical biology approach, providing a system that could facilitate the elucidation of the mechanism of WAAL-catalyzed ligation reaction.
O-antigen polymerase adopts a distributive mechanism for lipopolysaccharide biosynthesis
TLDR
This work presents the first biochemical evidence that Wzy functions in a distributive manner, and chemo-enzymatically synthesized the substrates of Wzy, the lipid-PP-linked repeat units.
Altered architecture of substrate binding region defines the unique specificity of UDP‐GalNAc 4‐epimerases
TLDR
This study provides a platform for further engineering of the UDP‐hexose 4‐epimerases, leads to a deeper understanding of the LPS biosynthesis and carbohydrate recognition by proteins, and may also have implications in development of novel antibiotics and more economic synthesis of UDP‐GalNAc and downstream products such as carbohydrate based vaccines.
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