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The temporal and spatial control over the delivery of materials such as siRNA into cells remains a significant technical challenge. We demonstrate the pulsed near-infrared (NIR) laser-dependent release of siRNA from coated 40 nm gold nanoshells. Tat-lipid coating mediates the cellular uptake of the nanomaterial at picomolar concentration, while(More)
An elusive goal for systemic drug delivery is to provide both spatial and temporal control of drug release. Liposomes have been evaluated as drug delivery vehicles for decades, but their clinical significance has been limited by slow release or poor availability of the encapsulated drug. Here we show that near-complete liposome release can be initiated(More)
BACKGROUND & AIMS During stress, erosion of protective intestinal mucus occurs in association with adherence to and disruption of the intestinal epithelial barrier by invading opportunistic microbial pathogens. The aims of this study were to test the ability of a high-molecular-weight polyethylene glycol compound, polyethylene glycol 15-20, to protect the(More)
An intact cell membrane serves as a barrier, controlling the traffic of materials going into and out of the cell. When the integrity of the membrane is compromised, its transport barrier function is also disrupted, leaving the cell vulnerable to necrosis. It has been shown that triblock copolymer surfactants can help seal structurally damaged membranes,(More)
The interaction between lipid bilayers and poloxamers has recently attracted much attention, and contradicting effects of poloxamers have been revealed on the integrity of lipid membranes; poloxamers are reported to be either effective sealants or permeabilizers of cell membranes depending on the cell type. To elucidate poloxamer-membrane interactions, we(More)
P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) is known as an effective membrane sealant. Using a dipalmitoylphosphatidylcholine monolayer at the air-water interface to mimic the outer leaflet of the cell membrane, we have examined the interaction between P188 and a phospholipid film.(More)
The lipid membrane has been shown to mediate the fibrillogenesis and toxicity of Alzheimer's disease (AD) amyloid-beta (Abeta) peptide. Electrostatic interactions between Abeta40 and the phospholipid headgroup have been found to control the association and insertion of monomeric Abeta into lipid monolayers, where Abeta exhibited enhanced interactions with(More)
The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack(More)
OBJECTIVE To investigate the effect of topical application of leptin in promoting burn wound healing in rats. METHODS Four parallel second-degree burn wounds induced on the back of 18 Wistar rats were divided into leptin treatment group (treated with topical application of 400 ng/ml leptin dissolved in PBS) and control group (treated with PBS). The time(More)