Guohua Guan

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BACKGROUND Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources(More)
BACKGROUND Lipolytic enzymes are commonly used to produce desired flavors in lipolyzed milkfat (LMF) manufacturing processes. However, the choice of enzyme is critical because it determines the final profile of fatty acids released and the consequent flavor of the product. We previously constructed a metagenomic library from marine sediments, to explore the(More)
BACKGROUND We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the enzyme activity and amount of enzyme produced were not(More)
An extremely thermostable xylanaseB gene from hyperthermophilic bacterium Thermotoga maritima was ligated to pGHE vector and expressed in filamentous fungus Aspergillus niger. The vector was constructed using endogenous strong gla promoter, signal sequence, and pro-sequence (NVISKR). Positive transfor-mants of the expression vector secreted XynB with(More)
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