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A version of modified particle swarm optimization (PSO) algorithm has been proposed. The PSO algorithm has been modified to adopt to the discrete combinatorial optimization problem and reduce the probability of sinking into local optima. In the modified PSO algorithm, the velocity represents the probability of element in each particle taking value 1 or 0.(More)
The extracellular polymeric substances (EPS) of P. chrysosporium and their effects on Pb2+ biosorption were studied. The product, composition of EPS and the effects on Pb2+ biosorption capacity were investigated in lab via flask experiments. The surface changes of mycelium before and after EPS extraction, before and after Pb2+ adsorption were researched by(More)
A novel system for the detection of DNA hybridization in a homogeneous format is developed. This method is based on fluorescence quenching by gold nanoparticles used as both nanoscaffolds for the immobilization of capture sequences and nanoquenchers of fluorophores attached to detection sequences. The oligonucleotide-functionalized gold nanoparticles are(More)
A novel strategy for fabricating horseradish peroxidase (HRP)-based H(2)O(2) sensor has been developed by combining the merits of carbon sol-gel supporting matrix and nano-scaled particulate gold (nano-Au) mediator. The thiol functional group-derived carbon ceramic electrode (CCE) was first constructed using (3-mercaptopropyl) trimethoxy silane as sol-gel(More)
A universal approach is proposed in this study for the development of an aptameric assay system for proteins based on aptamer structure-switching-triggered ligation-rolling circle amplification (L-RCA) upon target binding. The strategy chiefly depends on the competition for binding the aptamer probe between target protein and a complementary single-stranded(More)
Picloram, a widely used chlorinated herbicide, is quite persistent and mobile in soil and water with adverse health and environmental effects. It is essential to establish a rapid and sensitive method for accurate detection of trace picloram in agricultural samples. We employed a disposable, nontoxic, and conductive chitosan/gold nanoparticles composite(More)
CeO(2)/Chitosan (CHIT) composite matrix was firstly developed for the single-stranded DNA (ssDNA) probe immobilization and the fabrication of DNA biosensor related to the colorectal cancer gene. Such matrix combined the advantages of CeO(2) and chitosan, with good biocompatibility, nontoxicity and excellent electronic conductivity, showing the enhanced(More)
A silver-enhanced colloidal gold metalloimmunoassay has been proposed for the determination of Schistosoma japonicum antibody (SjAb) in rabbit serum. The adult worm antigen of S. japonicum (SjAg) was adsorbed passively on the walls of a polystyrene microwell and then reacted with the desired SjAb. The colloidal gold-labeled goat anti-rabbit IgG secondary(More)
Aptamer-based rolling circle amplification (aptamer-RCA) was developed as a novel versatile electrochemical platform for ultrasensitive detection of protein. This method utilized antibodies immobilized on the electrode surface to capture the protein target, and the surface-captured protein was then sandwiched by an aptamer-primer complex. The aptamer-primer(More)
A new fluorescence method based on aptamer-target interactions has been developed for cocaine detection with target-induced strand displacement. Here we describe new probes, the hairpin-probe and the single strand-probe (ss-probe), that possess two recognition sequences of cocaine aptamer. In the presence of cocaine, both probes would associate with the(More)