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The electron microscope has been used to determine the weight distribution of isolated subcellular particles from normal rat liver. The following results are reported: (1) There exist at least two well defined weight populations of subcellular particles; their respective median weights are 1.3 x 10(-14) and 11 x 10(-14) gm. The lighter fraction is… (More)
A B S T R A C T The electron microscope has been used to determine the characteristic dimensions and the distribution of the dry mass in bull spermatozoa. The most important result is that all characteristic data are occurring in logarithmic distributions. Furthermore, no correlation between such parameters as head weight and tail weight or head length and… (More)
Dry mass of herpes simplex virus particles was measured by quantitative electron microscopy after isolation by surface spreading and critical-point drying of infected cells. The core weighed about 2 x 10(-16) gram, the empty naked capsid 5 x 10(-16) gram, the full naked capsid 7 x 10(-16) gram, and the enveloped nucleocapsid 13 x 10(-16) gram.
The dry mass of individual vaccinia virions, as an example of a presumably uniform biological population, prepared in different lost at the Institute for Tropical Hygiene in Hamburg, was tested for variability by quantitative electron microscopy. A value of 5.26 X 10(-15) g for the median weight of the particle was calculated from 7,300 determinations. By… (More)
A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was fairly homogeneous within the chromatids… (More)